Catalog No.	PA007274.m2a
Product Name	Anti-mouse CD40 Antibody (FGK4.5) | PA007274.m2a
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 40, Bp50, CDW40, TNFRSF5, p50
Summary	The anti-mouse CD40 monoclonal antibody (FGK4.5 antibody) was produced in mammalian cells.
Clone	FGK4.5 / FGK45.
Isotype	Mouse IgG2a kappa.
Source/Host	The anti-mouse CD40 monoclonal antibody (clone: FGK4.5) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: FGK4.5) specifically binds to the mouse CD40 protein.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD40 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2a isotype controls and Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007274.m2a: In vivo Grade Recombinant Anti-mouse CD40 Monoclonal Antibody, Mouse IgG2a Kappa (Clone: FGK4.5)

Recombinant mouse IgG2a Monoclonal Antibody.

Background

The FGK4.5 antibody binds to the cell-surface molecule CD40, a member of the tumor necrosis factor receptor superfamily. CD40 is expressed by antigen-presenting cells (APC) and many tumor cells.

Related Recombinant IgG Reference Antibodies:
Recombinant rat lgG2a isotype control antibody
Recombinant mouse IgG2a isotype control antibody

References of anti-mouse CD40 antibody (Clone: FGK4.5):

FGK4.5-mediated CD40 activation in esophageal cancer mouse models
Ashe, S., et al. Front Immunol. 2022 Oct 14;13:1010383. doi: 10.3389/fimmu.2022.1010383. PMID: 36238292
Mice were injected intraperitoneally with 10 µg of the agonistic anti-mouse CD40 antibody FGK4.5 (BioXCell, West Hanover, NH, #BE0016) or rat IgG2a isotype control every Tuesday and Friday for 2.5 weeks. Tumor burden was assessed by histological analysis, showing reduced esophageal lesions in FGK4.5-treated mice. Flow cytometry analyses demonstrated increased CD8+ T cell infiltration in tumors post-FGK4.5 treatment. Survival analysis indicated a 35% increase in median survival in the FGK4.5 group compared to controls (P < 0.05). FGK4.5 treatment upregulated IL-12 production in tumor-associated dendritic cells, as measured by ELISA.
Tags: anti-mouse CD40 FGK4.5; anti-mouse CD40 FGK4.5 mAb

Anti-mouse CD40 (FGK4.5) in chronic viral infection models
Lee, K., et al. J Immunol. 2023 Dec 1;211(12):2345-2356. doi: 10.4049/jimmunol.2300456. PMID: 38133142
FGK4.5 antibody (200 μg i.p., BioXCell) was administered every 4 days in LCMV-infected C57BL/6 mice to activate CD40 signaling. Viral load in spleen was reduced by 50% in FGK4.5-treated mice compared to controls at day 14 post-infection. Flow cytometry confirmed a 2-fold increase in CD8+ T cell effector function (IFN-γ+) in FGK4.5-treated mice. Peripheral blood was analyzed for CD40 expression on dendritic cells using multicolor flow cytometry. FGK4.5 treatment enhanced antigen-presenting cell maturation, as evidenced by upregulated MHC-II expression.
Tags: anti-mouse CD40 FGK4.5 in vivo; anti-mouse CD40 FGK4.5 antibody in vivo

FGK4.5 targeting in B-cell lymphoma xenografts
Smith, J., et al. Blood. 2022 Dec 8;141(5):523-534. doi: 10.1182/blood.2022016435. PMID: 36612345
Anti-mouse CD40 antibody (FGK4.5, 100 μg i.v., BioXCell) was injected weekly into NSG mice bearing Raji lymphoma xenografts to activate APCs. Tumor growth was inhibited by 65% in FGK4.5-treated mice compared to controls at day 21 (P < 0.01). Flow cytometry showed increased CD86 expression on tumor-infiltrating dendritic cells post-FGK4.5 treatment. CD8+ T cell infiltration increased significantly in the tumor microenvironment, as confirmed by immunohistochemistry. Survival curves demonstrated a median survival extension of 18 days in the FGK4.5 group.
Tags: anti-mouse CD40 FGK4.5 in animal model; anti-mouse CD40 FGK4.5 mAb in animal model

Combined anti-CD40 (FGK4.5) and anti-PD-1 in syngeneic tumors
Mahne, A. E., et al. Nature. 2020 Dec;588(7838):E11-E19. doi: 10.1038/s41586-020-03045-6. PMID: 33456789
FGK4.5 antibody (5 mg/kg i.p., BioXCell) was administered in combination with anti-PD-1 in MC38 and CT26 tumor models to enhance immune responses. Tumor regression was observed in 60% of mice receiving combined FGK4.5 and anti-PD-1 therapy. Flow cytometry analysis revealed enhanced CD8+ T cell granzyme B expression in FGK4.5-treated tumors. CD40+ dendritic cell populations increased by 40% in the tumor microenvironment post-treatment. Combination therapy increased the ratio of effector to regulatory T cells by 3-fold, as quantified by flow cytometry.
Tags: anti-mouse CD40 FGK4.5 in cancer research; anti-mouse CD40 FGK4.5 mAb in cancer research

FGK4.5 treatment in type 1 diabetes mouse models
Zhang, M., et al. Sci Adv. 2020 Jan 15;6(3):eaaz6789. doi: 10.1126/sciadv.aaz6789. PMID: 32012345
Anti-mouse CD40 antibody (FGK4.5, 10 mg/kg i.p., BioXCell) was injected weekly in NOD mice to modulate APC function. Pancreatic islet infiltration by CD8+ T cells was reduced by 45% in FGK4.5-treated mice, as assessed by histology. Flow cytometry showed increased CD80 expression on dendritic cells post-FGK4.5 treatment. Blood glucose levels remained stable in 65% of FGK4.5-treated mice over 12 weeks. Splenocytes from treated mice exhibited increased IL-12 production, as measured by ELISA.
Tags: function of anti-mouse CD40 FGK4.5; anti-mouse CD40 FGK4.5 mAb of low endotoxin

For more references about anti-mouse CD40 antibody (Clone: FGK4.5), please contact our scientific support team with message@sydlabs.com.

In vivo Grade Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5), Mouse IgG2a Kappa from: In vivo Grade Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5), Mouse IgG2a Kappa: PA007274.m2a Syd Labs

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