Catalog No.	PA007485.h1Fs
Product Name	Recombinant Anti-mouse CD11c Monoclonal Antibody (Clone: N418), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	αX integrin, CR4, p150, integrin alpha X
Clone	N418.
Isotype	Human IgG1 kappa.
Source/Host	The anti-mouse CD11c monoclonal antibody (clone: N418) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant N418 antibody for Flow Cytometry specifically binds to mouse CD11c.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-mouse CD11c Monoclonal Antibody (Clone: N418) for Flow Cytometry, Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007485.h1Fs: Recombinant Anti-mouse CD11c Monoclonal Antibody (Clone: N418) for Flow Cytometry, Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.

References of Anti-Mouse CD11c Monoclonal Antibody (Clone: N418)

1. Rapid monoclonal antibody generation via dendritic cell targeting in vivo
Licea, et al.Hybrid Hybridomics. 2003 Feb;22(1):23-31. PMID: 12713687
“To address this question we immunized mice with IgG-peptide conjugates prepared with the hamster anti-murine CD11c MAb N418. …Synthetic peptides corresponding to two different exposed regions of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a human C-type lectin, were conjugated to N418 using thiol-based chemistry. …The N418 MAb served as the targeting molecule and synthetic peptides as the Ag (MAb-Ag). …A rapid and peptide specific serum IgG response was produced by Day 7 when the synthetic peptides were linked to the N418 MAb, compared to peptide co-delivered with the N418 without linkage. …To address this question we immunized mice with IgG-peptide conjugates prepared with the hamster anti-murine CD11c MAb N418.”

2. Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization
Wang, H., et al.Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):847-52. PMID: 10639168
“Although the exact cellular role of CD11c is unclear, binding of mAb N418 has been demonstrated not to interfere with the antigen presentation process of dendritic cells (15) and has been reported to induce an antibody response to itself after a single inoculation. …Thus, mAb N418 is an excellent candidate for antigen delivery. …The reporter protein antigen we chose is a polyclonal goat anti-hamster IgG antibody, which binds well to mAb N418 and is a foreign protein to the mouse. …To determine whether antibody responses can be enhanced by targeting dendritic cells with the hamster mAb N418, immunizations were performed with goat anti-hamster IgG antibody as the model antigen. …The specificity and potential efficacy of this approach were evaluated by comparing separate groups of mice immunized with either a mixture of mAb N418 and goat anti-hamster IgG antibody or a control immunogen.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD11c Monoclonal Antibody (Clone: N418) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD11c monoclonal antibodies:

Recombinant Anti-mouse CD11c monoclonal antibody (Clone: N418)

Anti-mouse CD11c Antibody (N418) for Flow Cytometry from: Recombinant Anti-mouse CD11c Monoclonal Antibody (Clone: N418), Human IgG1 Fc Silent Kappa: PA007485.h1Fs Syd Labs