Catalog No.	PA007390.m1
Product Name	In vivo Grade Recombinant Anti-mouse CD11c Monoclonal Antibody, Mouse IgG1 Kappa (Clone: N418)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	X integrin, CR4, p150, integrin alpha X
Clone	N418.
Isotype	Mouse IgG1 Kappa.
Specificity/Sensitivity	The in vivo grade recombinant hamster monoclonal antibody (clone: N418) specifically binds to mouse CD11c.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11c protein.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In vivo Grade Recombinant Anti-mouse CD11c Monoclonal Antibody, Mouse IgG1 Kappa (Clone: N418) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007390.m1: Recombinant Anti-mouse CD11c Monoclonal Antibody(Clone: N418), Mouse IgG1 Kappa, In vivo Grade

Background

The hamster anti-mouse CD11c monoclonal antibody N418 (hamster IgG2 kappa) reacts with the mouse CD11c protein, the most widely used defining marker for dendritic cells (DCs). The CD11c protein helps cells stick together and is involved in many processes, including phagocytosis, cell migration, and the activation of T cells. CD11c helps cells stick together by binding to molecules like ICAM-1, fibrinogen, and iC3b, helps cells engulf and digest other cells, helps Langerhans cells activate T cells, regulates neutrophil maturation and function, and helps dendritic cells capture and activate other cells. In addition to dendritic cells and tissue macrophages, CD11c is expressed in different B-cell lymphomas, including chronic lymphocytic leukemia. CD11c-expressing cells are also found in the mouse nervous system.

Our recombinant N418 antibodies have a part (variable regions) or complete amino acid sequences of the hamster anti-mouse CD11c monoclonal antibody (hybridoma clone name or number: N418).

References of Anti-Mouse CD11c Monoclonal Antibody (Clone: N418)

1. Altered function and differentiation of age-associated B cells contribute to the female bias in lupus mice
Ruifeng Lai, et al.Cell Death Dis. 2021 Aug 10;12(8):780. doi: 10.1038/s41419-021-04063-w. PMID: 34376646; PMCID: PMC8355159
“Age-associated B cells (ABCs), characterized by the expression of CD11c and T-bet, accumulate in autoimmune diseases. This study demonstrates that the altered function and differentiation of ABCs contribute significantly to the female bias in lupus-prone mice. Understanding the role of CD11c+ B cells provides critical insights into the pathogenesis and therapeutic targeting of systemic lupus erythematosus.”

2. Dendritic Cells and Microglia Have Non-redundant Functions in the Inflamed Brain with Protective Effects of Type 1 cDCs
Chotima Böttcher, et al.Immunity. 2020 Nov 17;53(5):1038-1053.e9. doi: 10.1016/j.immuni.2020.10.003. PMID: 33113344; PMCID: PMC7578563
“Dendritic cells and microglia play distinct, non-redundant roles in regulating neuroinflammation. This study characterizes brain-resident CD11c+ classical dendritic cells (cDCs) and microglia during inflammatory states. The findings reveal that type 1 cDCs exert protective immune effects in the inflamed central nervous system, highlighting their potential as therapeutic targets.”

3. Anti-Mouse CD83 Monoclonal Antibody Targeting Mature Dendritic Cells Provides Protection Against Collagen Induced Arthritis
Xiaofeng Li, et al.Front Immunol. 2022 Feb 10;13:836488. doi: 10.3389/fimmu.2022.836488. PMID: 35222441; PMCID: PMC8866188
“Mature dendritic cells (DCs) are central regulators of autoimmune responses in rheumatoid arthritis. This study demonstrates that therapeutic administration of an anti-mouse CD83 monoclonal antibody selectively targets mature CD11c+ DCs to provide protection against collagen-induced arthritis. These results suggest that targeting DC activation pathways represents a highly effective immunotherapeutic strategy for treating autoimmune joint inflammation.”

4. Dynamics of host immune response development during Schistosoma mansoni infection
Fiona M. S. J. van den Biggelaar, et al.PLoS Negl Trop Dis. 2022 Aug 9;16(8):e0010651. doi: 10.1371/journal.pntd.0010651. PMID: 35943960; PMCID: PMC9362740
“Host immune responses undergo complex temporal dynamics during progressive parasitic infections. This study tracks the initiation and development of immune reactions during Schistosoma mansoni infection in mouse models. The analysis highlights the pivotal role of CD11c+ myeloid populations in driving antigen presentation and shaping the downstream adaptive immune landscape.”

5. CD11c+CD88+CD317+ myeloid cells are critical mediators of persistent CNS autoimmunity
Jonathan P. S. Knier, et al.J Exp Med. 2021 May 3;218(5):e20201111. doi: 10.1084/jem.20201111. PMID: 33818556; PMCID: PMC8040603
“Persistent neuroinflammation in autoimmune diseases requires continuous reactivation of encephalitogenic T cells. This study identifies a unique subset of CD11c+ CD88+ CD317+ myeloid cells as key drivers of chronic central nervous system autoimmunity. This distinct cell population accumulates in inflamed tissues and exhibits superior antigen-presenting capabilities that sustain local T-cell responses.”

For more technical references or data sheets regarding the Anti-Mouse CD11c Monoclonal Antibody (Clone: N418) formats, please contact our scientific support team at message@sydlabs.com.

Anti-mouse CD11c Antibody (N418) from: In vivo Grade Recombinant Anti-mouse CD11c Monoclonal Antibody, Mouse IgG1 Kappa (Clone: N418): PA007390.m1 Syd Labs