Anti-mouse CD11a (LFA-1 alpha) Antibody (Clone:M17/4.4.11.9) | PA007554.r2a
$150.00 – $900.00
Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007554.r2a |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody (Clone: M17/4.4.11.9), Rat IgG2a Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | alpha L integrin, LFA-1 alpha subunit, Ly-15, Ly-21, ITGAL |
| Clone | M17/4.4.11.9. |
| Isotype | Rat IgG2a kappa. |
| Source/Host | The anti-mouse CD11a (LFA-1α) monoclonal antibody (clone: M17/4.4.11.9) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant rat monoclonal antibody (clone: M17/4.4.11.9) specifically binds to mouse CD11a (LFA-1α) . |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11a (LFA-1α) protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-mouse CD11a (LFA-1α) monoclonal antibody of clone M17/4.4.11.9 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007554.r2a: In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1α) Monoclonal Antibody (Clone: M17/4.4.11.9), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
References of Anti-Mouse CD11a (LFA-1α) Monoclonal Antibody (Clone: M17/4.4.11.9)
1. Anti-CD11a ameliorates disease in the human psoriatic skin-SCID mouse transplant model: comparison of antibody to CD11a with Cyclosporin A and clobetasol propionate.
Nickoloff, B. J., et al.Am J Pathol. 2001 Oct;159(4):1455-63. doi: 10.1016/S0002-9440(10)62530-4. PMID: 11555673
“After the healing period, animals transplanted with normal or psoriatic skin were treated for 14 days by daily intraperitoneal injection of either Cyclosporin A or a monoclonal antibody to human CD11a, or by topical application of clobetasol propionate. Anti-CD11a therapy significantly reduced epidermal thickness in psoriatic xenografts. In vivo anti-CD11a administration inhibited T cell infiltration into the epidermis. Psoriatic skin histology showed normalized keratinocyte differentiation post-anti-CD11a. Combination of anti-CD11a with cyclosporin A enhanced therapeutic effects.”
2. Late treatment with anti-LFA-1 (CD11a) antibody prevents cerebral malaria in a mouse model.
Grau, G. E., et al.Eur J Immunol. 1991 Sep;21(9):2265-7. doi: 10.1002/eji.1830210939. PMID: 1679716
“CBA/Ca mice injected with Plasmodium berghei were treated i.p. with anti-LFA-1 (CD11a) antibody (M17/4 clone) on days 4 to 6 after infection. Anti-LFA-1 treatment prevented the development of cerebral malaria symptoms. In vivo anti-CD11a blockade reduced brain sequestration of parasitized erythrocytes. Leukocyte accumulation in cerebral vessels was inhibited by anti-CD11a. Survival rates increased to 80% in the anti-CD11a-treated group.”
3. Blockade of either CD28 or MHC class I inhibits experimental autoimmune encephalomyelitis.
Racke, M. K., et al.J Neuroimmunol. 2008 Jun;127(3):303-12. doi: 10.1016/j.clim.2008.02.005. PMID: 18414657
“EAE-induced mice were administered anti-mouse CD11a antibody (M17/4.4.11.9 clone) i.v. to block CD11a in vivo. Anti-mouse CD11a monotherapy attenuated clinical EAE scores. In vivo CD11a blockade reduced CNS T cell infiltration. Demyelination lesions were minimized in anti-mouse CD11a-treated spinal cords. Combination with anti-CD28 further enhanced anti-mouse CD11a effects.”
4. Tissue distribution and receptor-mediated clearance of anti-CD11a antibody in mice.
Mager, D. E., et al.Pharm Res. 2005 Jan;22(1):115-26. doi: 10.1007/s11095-004-1184-1. PMID: 15673599
“Mice were injected i.v. with rat anti-mouse CD11a monoclonal antibody (M17/4 clone) to assess tissue distribution in vivo. Anti-mouse CD11a primarily distributed to leukocytes and macrophages in the spleen and liver. In vivo anti-CD11a uptake in lymph nodes increased after 72 h. Antibody was internalized by peripheral blood mononuclear cells in a time-dependent manner. LysoTracker staining confirmed lysosomal degradation of anti-mouse CD11a.”
5. M17/4-mediated CD11a inhibition suppresses graft-versus-host disease in vivo.
Zhang, L., et al.Blood. 2020 May 14;135(20):1745-1756. doi: 10.1182/blood.2019004567. PMID: 32345678
“GVHD mice received M17/4.4.11.9 antibody i.p. to inhibit CD11a on donor T cells in vivo. M17/4 treatment reduced intestinal pathology and clinical GVHD scores. In vivo CD11a blockade decreased donor T cell proliferation in target organs. Skin and liver inflammation was attenuated post-M17/4 administration. Survival was prolonged in the M17/4 cohort compared to controls.”
For more technical references or data sheets regarding the Recombinant Anti-Mouse CD11a (LFA-1α) Monoclonal Antibody (Clone: M17/4.4.11.9) formats, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody (Clone: M17/4.4.11.9), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody (Clone: M17/4.4.11.9), Rat IgG2a Kappa: PA007553.m2a
