Catalog No.	PA007278.m2a
Product Name	Anti-Human CD56 (N901) Antibody | PA007278.m2a
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Neural cell adhesion molecule, NCAM, CD56, MSK39, neural cell adhesion molecule 1, NCAM1
Clone	N901
Isotype	Mouse IgG2a kappa
Source/Host	The anti-human CD56 monoclonal antibody (clone: N901) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: N901) specifically binds to the human CD56 protein.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD56 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The In vivo Grade Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG1 Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007278.m2a: Recombinant Anti-human CD56 Antibody (Clone: N901), Mouse IgG2a Kappa, In vivo Grade

Recombinant mouse IgG2a Monoclonal Antibody.

Background of Anti-human CD56 Antibody (Clone: N901)

The recombinant N901 antibody binds to the human CD56 protein, a homophilic binding glycoprotein expressed on the surface of neurons, glia and skeletal muscle. CD56 is a marker of neural lineage commitment. CD56 expression is found in the hematopoietic system, natural killer cells, and other lymphoid cells, including gamma delta (γδ) Τ cells and activated CD8+ T cells, as well as on dendritic cells. CD56 has a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

References of Anti-human CD56 Monoclonal Antibody:

1、PD1 blockade enhances cytotoxicity of in vitro expanded natural killer cells towards myeloma cells.
Guo, Y., et al. Oncotarget. 2016 Jul 26;7(30):48360-48374. doi: 10.18632/oncotarget.10235. PMID: 27356741
“With this protocol using anti-human CD16 antibody and interleukin (IL)-2, NK (CD3-CD56+) cells could be expanded about 4000-fold with over 70% purity during a 21-day culture. …The expanded NK (exNK) cells were shown to be highly cytotoxic to multiple myeloma (MM) cells (RPMI8226) at low NK-target cell ratios. …Apparently, developing a protocol for efficient activation and expansion of NK cells to achieve sufficient number of functional NK cells is curial for adoptive allo- and auto-NK cell therapies. …Targeting on immune checkpoint molecules such as programmed cell death protein 1 (PD1) and its ligands PD-L1 and PD-L2 by antibodies to block their inhibitory signaling has achieved great success in treatment of several solid tumors and hematological malignancies. …Compared with the exNK cells without PD1 blocking in expansion system, our results showed that the exNK-PD1 blockage presented a significant higher percentage of degranulated (CD3-CD56+CD107a+) cells (64.0%±1.0% vs. 53.1%±2.7%, P<0.05).”

2、Immunohistochemical observation of local inflammatory cell infiltration in the host-tissue reaction site of human hydatid cysts.
Jafari, R., et al. J Helminthol. 2019 May;93(3):277-285. doi: 10.1017/S0022149X1800024X. PMID: 29708080
“The aim of this study was to evaluate the pattern of local immune cell infiltration in human cystic echinococcosis (CE) by identifying the subtypes of immune cells using immunohistochemistry (IHC). …Fifty surgically removed hydatid cyst samples and surrounding tissues were collected from patients referred to Al-Zahra Hospital, Isfahan, Iran. …IHC was performed on the surrounding host tissue of hydatid cysts using anti-human CD3, CD19, CD8, CD4, CD68, CD56, Ki-67 and Foxp3 (forkhead box P3) antibodies. …The results were then compared to hepatocellular carcinoma and chronic hepatitis. …However, in some cases there were no positive cells for CD56+ natural killer cells and Foxp3+ regulatory T cells.”

3、Analysis of main T-cell subsets and activated T supressor/cytotoxic cells in patients with Borrelia burgdorferi s. lato only infection and co-infections with Anaplasma phagocytophilum, Bartonella spp. and Babesia microti.
Tomasiewicz, K., et al. Ann Agric Environ Med. 2016;23(1):111-5. doi: 10.5604/12321966.1196864. PMID: 27007527
“Directly conjugated anti-human monoclonal antibodies against CD3, CD4, CD8, CD16, CD56, HLA-DR and CD69 were used. …No significant differences were observed with respect to thepretreatment level of CD4+ and CD8+ cells. …Co-infections with Anaplasma phagocytophilum, Bartonella spp. and Babesia microti had no impact on T-cell percentages in Lyme disease patients. …The study was designed to assess the role of some important immunologic factors with regards to both laboratory results and clinical symptoms in patients with confirmed Lyme disease. …In patients with symptoms relief and symptoms persistence, lower percentages of CD4+ and CD8+ cells were found, but with no statistical dependence.”

For more references about Anti-human CD56 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Anti-human CD56 (N901) from: Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG2a Kappa: PA007278.m2a Syd Labs

No more offers for this product!