Catalog No.	PA007703.h1
Product Name	Anti-FITC Monoclonal Antibody (Clone: 4M5.3) | PA007703.h1
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Anti-FITC Antibody, Anti-fluorescein Isothyocianate Antibody
Gene ID	https://www.ushelf.com/wp-content/themes/porto/inc/admin/theme_options/products/full_width.jpg
Clone	4M5.3.
Isotype	Human IgG1 Kappa.
Source/Host	The recombinant anti-FITC monoclonal antibody (clone: 4M5.3) was produced in mammalian cells.
Specificity/Sensitivity	The recombinant anti-FITC monoclonal antibody (clone: 4M5.3) specifically binds to FITC.
Applications	ELISA, Western Blot, Flow Cytometry.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The recombinant anti-FITC monoclonal antibody of clone 4M5.3 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007703.h1: Recombinant Anti-FITC Monoclonal Antibody (Clone: 4M5.3), Human IgG1 Kappa

Recombinant human IgG1 monoclonal antibody.

Background of Anti-FITC Monoclonal Antibody

Recombinant mouse anti-FITC antibodies recognize FITC attached to proteins, peptides, nucleic acids, or solid matrices, and can be offered in fluorophore and enzyme-conjugated versions.

The anti-FITC monoclonal antibody can be used as a second-step reagent in various applications for detecting FITC (fluorescien)-conjugated targets. The FITC-anti-FITC system is widely used in immunoassay signal amplification techniques because an anti-FITC antibody may bind FITC (fluorescien) with extremely high affinity.

The FITC-anti-FITC system is different from the biotin-streptavidin system because biotin is naturally present in a large variety of biological samples, and it may affect the results of biotin-based assays, such as reduced sensitivity, false negatives or false positives. FITC (Fluorescien) is not naturally present in any biological samples.

Please contact us to ask for a quote for the engineered anti-FITC antibody mutants of various isotypes, and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated antibodies and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-FITC antibodies and mutants are available.

References of Anti-FITC Monoclonal Antibody:

Antigen-antibody interactions and structural flexibility of a femtomolar-affinity antibody
Fukunishi H, et al. Biochemistry.2012 Mar 27;51(12):2597-2605. doi: 10.1021/bi3000319. PMID: 22390639
The femtomolar-affinity mutant antibody (4M5.3) generated by directed evolution is interesting because of the potential of antibody engineering. In this study, the mutant and its wild type (4-4-20) were compared in terms of antigen-antibody interactions and structural flexibility to elucidate the effects of directed evolution. The pulling forces of SMD simulations elucidated the regions that form strong attractive interactions in the binding pocket. These landscapes were regarded as funnel-like binding free energy landscapes. It was shown that the fluctuations in the positions of the antigen and residues in contact with antigen tend to be smaller in the mutant than in the wild type.
Tags: activity of Anti-FITC Monoclonal; Anti-FITC Monoclonal

Rapid refolding and polishing of single-chain antibodies from Escherichia coli inclusion bodies
Jessica R Sinacola?et al. Protein Expr Purif. 2002 Nov;26(2):301-8. doi: 10.1016/s1046-5928(02)00538-7. PMID: 12406685
Two anti-fluorescein single-chain antibodies, 4-4-20 and 4M5.3, were expressed as inclusion bodies in E. coli for use in a comparative refolding study. Although all three methods produced active protein for both 4-4-20 and 4M5.3, the extent of aggregation differed greatly among the methods. Genes for the anti-fluorescein single-chain antibodies 4-4-20 and 4M5.3 in pRS316 [17] were subcloned into pET-21a (Novagen) using the NheI and XhoI restriction sites. A significant decrease in the fluorescent intensity of a fluorescein solution is observed upon the binding of fluorescein to the active site of 4-4-20 and 4M5.3. The anti-fluorescein single-chain antibodies 4-4-20 and 4M5.3 were each expressed as inclusion bodies in E. coli as described in the Materials and methods section.
Tags: Anti-FITC Monoclonal for cancer research; Anti-FITC Monoclonal for human tumor model

For more references about Anti-FITC Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-Biotin antibodies:

In Vivo Grade Recombinant Anti-Biotin Antibodies (Clone F1)

Syd Labs provides the following recombinant anti-Fluorescein/Digoxin/Biotin/Phospho-Tyrosine monoclonal antibodies:

Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4-4-20 (enhanced))
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4M5.3)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 5A7)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 26-10)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone M33)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone Py20)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone 4G10)

Recombinant Anti-FITC Monoclonal Antibody (Clone: 4M5.3), Human IgG1 Kappa from: Recombinant Anti-FITC Monoclonal Antibody (Clone: 4M5.3), Human IgG1 Kappa: PA007703.h1 Syd Labs

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