Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi

Catalog No.	BP003053
Product Name	Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	SARS-CoV-2 NP Protein, 2019-nCoV Nucleocapsid Protein, 2019-nCoV NP Protein, 2019-nCoV N protein, COVID-19 Virus N Protein
Source	recombinant SARS-CoV-2 nucleocapsid protein (Ser2-Ala419) was expressed in E.coli with a His tag and Avi at the C-terminus.
Formulation	The recombinant SARS-CoV-2 (2019-nCoV) nucleocapsid protein-His-Avi is supplied as 0.22 um filtered solution in 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5.
Purity	> 95% by PAGE under reduced condition.
Endotoxin	Less than 1 EU per ug by the LAL method.
Shipping	The recombinant SARS-CoV-2 (2019-nCoV) nucleocapsid protein-His-Avi is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Note	Great for research reagent and IVD material. SARS-CoV-2 nucleocapsid antibodies, immunoassay kits, custom antibody and kit development services are available. To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services
Order Offline	Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card.

Description

BP003053: Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi

Background

Source: recombinant SARS-CoV-2 nucleocapsid protein (Ser2-Ala419) was expressed in E.coli with a His tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 48.9 kDa.
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: The recombinant SARS-CoV-2 (2019-nCoV) nucleocapsid protein-His-Avi is supplied as 0.22 um filtered solution in 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5.
Purity: > 95% by PAGE under reduced condition.

Shipping: The recombinant SARS-CoV-2 (2019-nCoV) nucleocapsid protein-His-Avi is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.

Questions and answers:

Question: Are the nucleocapsid proteins between SARS-CoV-2 and SARS-CoV highly conserved? How about their epitope sequences?
Answer: The nucleocapsid protein from SARS-CoV shows a similarity with the SARS-CoV-2 homologue slightly above 90%. Comparison of the epitope sequences, AQFAPSASAFFGMSR, QFAPSASAFFGMSRIGM, PKGFYAEGSRGGSQASSR, YNVTQAFGRRGPEQTQGNF, and KHWPQIAQFAPSASAFF highlights that SARS-CoV scored 100% sequence identity with SARS-CoV-2 epitopes. It is the reason why the SARS-CoV nucleocapsid antibodies have been widely used in manufacturing of SARS-CoV-2 IgG/IgM antibody rapid test kits.

Question: Does it mean that there is false positive result if the nucleocapsid-based SARS-CoV-2 IgG/IgM antibody rapid test kit is used for clinical diagnostic test?
Answer: Yes, it is highly possible especially if the patient has been infected by other coronavirus(es).

Question: Then why are the nucleocapsid-based SARS-CoV-2 IgG/IgM antibody test kits still widely used?
Answer: The nucleocapsid protein is the most abundant protein in SARS-CoV-2 and is highly immunogenic, it is often used as a marker in diagnostic immunoassays in the ELISA and colloidal gold-based rapid test kits. Its sensitivity is high though crossactivity is also high.

Question: Do you have discount if we place bulk order of the recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid protein-His-Avi for IVD material?
Answer: Sure. We also sell monoclonal human and rabbit nucleocapsid antibodies as well as alpaca nucleocapsid VHH antibodies.

SARS-CoV-2 (2019 nCoV or COVID-19 virus) proteins:
Biotinylated Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-Fc-Avi
Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein
Recombinant SARS-CoV-2 Spike S-Fc-Avi Protein
Biotinylated Recombinant SARS Spike RBD-His-Avi
Recombinant SARS Spike RBD-His-Avi
Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi
Biotinylated Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-Fc-Avi Protein

To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services

The virus causing Coronavirus Disease 2019 (COVID-19) was named 2019 novel coronavirus (2019-nCoV) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Similar to those of other coronaviruses, there are four major structural proteins in SARS-CoV-2, S protein (spike protein), E protein (envelope protein), M protein (membrane protein), and N protein (nucleocapsid protein). The main function of Nucleocapsid protein is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells.

The nucleocapsid (N) phosphoprotein is the most abundant protein in coronavirus. Because the N protein is highly immunogenic and conserved among cornnaviruses, it is often used as a marker in diagnostic immunoassays in the ELISA and colloidal gold-based rapid test kits. However, positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as coronavirus HKU1, NL63, OC43, or 229E.

Please remember our product information: Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi: BP003053 Syd Labs

Additional information

Ship from Country	USA
Size	1 mg, 100 ug

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