Recombinant Human G-CSF Protein
$250.00 – $1,750.00
Syd Labs Recombinant Human G-CSF Protein BP000175-GD16, The ED50 of the 174-amino acid form of recombinant human G-CSF protein (Granulocyte Colony-Stimulating Factor, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF), calculated by the dose-dependant proliferation of murine NFS-60 indicator cells, is < 0.1 ng/ml, corresponding to a specific activity of 6.0x10^7 IU/mg.
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Catalog No. | BP000175-GD16 |
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Product Name | Recombinant Human G-CSF Protein |
Supplier Name | Syd Labs, Inc. |
Brand Name | Syd Labs |
Synonyms | Granulocyte colony-stimulating factor, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF |
Source | E. coli-derived. |
Formulation | In 0.2 um filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0. |
Purity | > 95% by SEC-HPLC and reducing SDS-PAGE. |
Endotoxin | < 0.01 ng/ug (0.1 IEU/ug). |
Shipping | The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below. |
Note | Conditions of optimal G-CSF protein performance should be determined experimentally by the investigator. |
Order Offline | Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card. |
Description
BP000175-GD16: Recombinant Human G-CSF Protein
Background
Human Granulocyte-Colony-Stimulating Factor (G-CSF), a 20 kDa glycoprotein containing internal disulfide bonds, induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. G-CSF functions through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT), Ras/mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. Bacterial endotoxins, TNF, Interleukin-1, and GM-CSF induce the synthesis of G-CSF while prostaglandin E2 inhibits its synthesis. Interleukin-17 induces the secretion of G-CSF in epithelial, endothelial, and fibroblastic cells.
BP000175-GD16: Recombinant Human Granulocyte colony-stimulating factor (G-CSF) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser- Phe-Leu-leu.
Predicted molecular mass: 20 kDa, the 174-amino acid form of human G-CSF.
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is < 0.1 ng/ml, corresponding to a specific activity of 6.0x10^7 IU/mg. Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.01 ng/ug (0.1 IEU/ug). Formulation: In 0.2 um filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0. Shipping: The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt if stored at 4°C as supplied. Other G-CSF Proteins:
Recombinant human Pegylated G-CSF protein
Recombinant human G-CSF protein with His-tag
Recombinant human G-CSF protein produced in HEK
Recombinant human G-CSF protein produced in CHO
Recombinant mouse G-CSF protein
There are two forms, 174- and 180-amino-acid, of the natural human G-CSF protein. The 174-amino acid form is more abundant and active than the longer form and widely used in research and therapeutics. The structure of the recombinant human G-CSF protein expressed in E. coli, such as filgrastim, differs slightly from those of the natural glycoprotein and the recombinant human G-CSF protein expressed in mammalian cells such as CHO cells. PEGylation (polyethylene glycol) significantly extends half-life of the recombinant human G-CSF protein.
G-CSF Antibodies:
G-CSF monoclonal antibody
G-CSF monoclonal antibody (FITC-conjugated)
G-CSF monoclonal antibody (Biotin-conjugated)
G-CSF ELISA Kits:
Human G-CSF ELISA kit
Mouse G-CSF ELISA kit
Please remember our product information: Recombinant Human G-CSF Protein: BP000175-GD16 Syd Labs
Additional information
Ship from Country | USA |
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Size | 300 ug, 1 mg, 50 ug |