Catalog No.	PA007395
Product Name	Purified Human IgG (Whole Molecule), Native (Clone: N/A) | PA007395
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Human Immunoglobulins, Human Fc Blocking Reagent
Summary	The purified IgG polyclonal antibody (whole molecule) was purified from a mixture of normal human serum.
Clone	N/A
Isotype	Human IgG
Specificity/Sensitivity	N/A
Applications	ELISA, multiplex and microarray assays, and human Fc blocking reagent for immunoassays including flow cytometry, IHC, IF, WB, and ELISA.
Form Of Antibody	Lyophilized, no stabilizers or preservatives.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 month from date of receipt, 2 to 8°C as supplied.
Note	Purified human IgG (whole molecule) from a mixture of normal human serum. A human Fc blocking reagent or a component of a human Fc blocking solution. Recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are available.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007411PA007395: Purified Human IgG (Whole Molecule), Native (Clone: N/A)

Native human IgG Polyclonal Antibody (whole molecule).

Background

The purified native human IgG (whole molecule) from a mixture of normal human serum can be used as a human Fc blocking reagent or a component of a human Fc blocking solution.

The most popular human Fc blocking reagents include:
Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls.
Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions.

Best choice of human Fc blocking reagents based on your application are:
For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

References of Purified Human IgG:

1、Prophylactic and therapeutic protection of human IgG purified from sera containing anti-exotoxin A titers against pneumonia caused by Pseudomonas aeruginosa.
Yu, B., Gu, H., et al. Hum Vaccin Immunother. 2019 ;15(12):2993-3002. doi: 10.1080/21645515.2019.1619404. PMID: 31116632.
“Thus, we purified the ETA-specific IVIGs from healthy donors in this study. …Following that, the neutralizing activities of the purified anti-ETA IgGs were evaluated in vitro. …However modest inflammatory responses, which were characterized by reduced neutrophil infiltration and interstitial edema, were observed in mice given high, medium and even low levels of human IgGs and rabbit IgGs. …The significance of the differences was determined by unpaired parametric tests (one-way ANOVA), which was followed by the Tukey’s HSD test for multiple comparisons. …All experiments except the animal challenge assays were conducted a minimum of three times.”

2、Purified complement C3b triggers phagocytosis and activation of human neutrophils via complement receptor 1.
Boero, E., et al. Sci Rep. 2023 Jan 6;13(1):274. doi: 10.1038/s41598-022-27279-4. PMID: 36609665.
“For the IgG surfaces, we labelled with 30 µg/mL anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (BioLegend), and for C3b surfaces we coated with 10 µg/mL anti-C3b antibody conjugated to FITC. To convert intensity values to actual molecular densities, we utilized the QSC calibration kit (Bangs Laboratories) which captures known amounts of antibody for each calibration bead, allowing us to construct a calibration curve of molecular density vs. …Here, we employ serum-free methods to couple purified complement C3b onto E. coli bacteria and beads and then expose human neutrophils to these C3b-coated targets. …We show that purified C3b can potently trigger phagocytosis and killing of bacterial cells via Complement receptor 1. …Our data reveal previously overlooked roles of C3b in phagocytosis, demonstrating that purified C3b drives bacterial uptake and killing via CR1 in a serum-free environment. …Having established that purified C3b can drive phagocytosis in neutrophils, we further studied the uptake mechanism.”

3、Affinity Purification of PfEMP1-Specific Antibodies from Human Blood.
Olsen, R. W., et al. Methods Mol Biol. 2022;2470:369-379. doi: 10.1007/978-1-0716-2189-9_27. PMID: 35881359.
“Purified human antibodies can be used, for example, to study the interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells, and to identify which IgG antibodies play a functional role in natural acquired immunity. …Acquired immunity against Plasmodium falciparum infections relies heavily on IgG antibodies specific for PfEMP1 proteins expressed on the surface of infected erythrocytes. …This chapter describe how to affinity purify PfEMP1-specific human antibodies on an affinity column coupled with PfEMP1 protein. …We include ELISA-based methods for identification of human plasma samples reactive against PfEMP1, and for testing of affinity purified IgG antibodies prior to their use in more advanced procedures. …This work was supported by Lundebeckfonden (R180-2014-3098 and R313-2019-322) and the Danish Council for Independent Research (8020-00034B).”

4、Low doses of IgG from atopic individuals can modulate in vitro IFN-γ production by human intra-thymic TCD4 and TCD8 cells: An IVIg comparative approach.
Sgnotto, F. R., et al. Hum Vaccin Immunother. 2017 Jul 3;13(7):1563-1572. doi: 10.1080/21645515.2017.1299299. PMID: 28441069.
“Based on the above evidence, the aim of this study was to evaluate the possible differential effects of purified IgG from atopic and non-atopic individuals on cytokine production by human intra-thymic αβT cells, especially IFN-γ production. …Time course of cell frequency and viability and of the effect of purified IgG on human intra-thymic αβT cells. …Next, we performed a comparative analysis of the effects of purified IgG that is used as an in vivo human therapy (IVIg) using a culture protocol that replicated our culture experiments. …Using a pool of purified human IgG antibodies (IVIg), which is routinely used in the treatment of patients with primary immunodeficiencies,Citation35 IgG was found to decrease IFN-γ levels in PBMC culture supernatants. …In conclusion, we present evidence of a possible novel mechanism of immune modulation mediated by human IgG.”

5、Flow cytometry analysis of anti-polyethylene glycol antibodies in human plasma.
Fang, J.-L., et al. Toxicol Rep. 2020 Dec 26;8:148-154. doi: 10.1016/j.toxrep.2020.12.022. PMID: 33437656.
“FITC-conjugated goat anti-human IgG (γ-chain specific) secondary antibody was purchased from Thermo Fisher Scientific (Waltham, MA). …After the beads were stained for bound IgG or IgM with a specific FITC-conjugated anti-human IgG or IgM secondary antibody, the beads were washed three times with PBS and analyzed on a Beckman Coulter CytoFlex flow cytometry (Indianapolis, IN). …The FITC-conjugated anti-human IgG and IgM secondary antibodies were affinity-purified and further tested by flow cytometry to ensure no cross-reaction with other human immunoglobulins. …Human IgG or IgM was then purified following the manufacturer’s instructions. …To confirm further the detection of anti-PEG IgG or IgM in healthy blood donors, Western blotting assay of purified plasma from human IgG and IgM purification columns was performed.”

For more references about Purified Human IgG please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following anti-human Fc blocking reagents:

Anti-human CD16 monoclonal antibody (Clone: 3G8)
Anti-human CD32 monoclonal antibody (Clone: IV.3)
Anti-human CD64 monoclonal antibody (Clone: 22)
Purified human IgG
Recombinant human IgG antibody mixes
Recombinant human IgG isotype control antibodies
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies

Syd Labs provides the following anti-mouse Fc blocking reagents:

Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies

Purified Human IgG (Whole Molecule), Native (N/A) from: Purified Human IgG (Whole Molecule), Native: PA007395 Syd Labs

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