Protein Expression in Mammalian Cells
We can optimize stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the methods of SDS-PAGE, Western, ELISA or functional analysis.
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| Catalog No. | BS005 |
|---|---|
| Product Name | Protein Expression in Mammalian Cells |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Summary | We can optimize stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the methods of SDS-PAGE, Western, ELISA or functional analysis. |
| Note | No DNAs using our recombinant antibody expression vectors will be delivered. |
| Order Offline | Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card. |
Description
Express your proteins with or without tags in bacterial, yeast, insect, or mammalian cells. We provide free consulting services for selecting expression vectors. If your proteins will be used for antibody development, we provide free consulting services for selecting protein fragments with higher immunogenicity and antigen specificity.
We can optimize stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the methods of SDS-PAGE, Western, ELISA or functional analysis. We optimize protein expression by modification of the following factors:
a. promoter
b. ribosome binding site
c. vector copy number
d. host cell
e. growth condition including media and additives induction, time, and temperature
Protein purification process can be optimized to meet different requirements:
a. Tag-based affinity purification of recombinant proteins, purity higher than 80%;
b. Purification with ion exchange and/or size exclusion chromatography, purity higher than 90-95%;
c. Customized purification methods for biological activities. If requested, in vitro biological activity will be checked.
Package 1: expression optimization
Package 2: small, middle or large scale expression
Package 3: purification optimization
Package 4: expression optimization + purification optimization
Package 5: small, middle or large scale purification
Package 6: DNA subcloning + expression optimization + purification optimization + purification
Package 7: DNA subcloning + expression optimization + purification optimization + purification (guaranteed)
Package 8: gene synthesis + DNA subcloning + expression optimization + purification optimization + purification
Package 9: gene synthesis + DNA subcloning + expression optimization + purification optimization + purification (guaranteed)
Additional information
| Ship from Country | USA |
|---|---|
| Size | 1 package |