D-Luciferin Potassium Salt (MB102)
$195.00 – $1,799.00
The cheapest D-Luciferin potassium salt in the market. High purity 99% for both in vitro luciferin luciferase ATP assay and in vivo mouse imaging. Quality of Syd Labs’s D-luciferin potassium salt has been proven by a number of publications citing the D-Luciferin. Validated by the worldwide scientists.
- Details & Specifications
- References
- More Offers
| Catalog No. | MB000102-R70170 |
|---|---|
| Product Name | D-Luciferin Potassium Salt (MB102) |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Summary | CAS No.: 115144-35-9 Molecular Formula: C11H7N2O3S2.K Molecular Weight: 318.42 Purity >99% (chemical and optical purity) |
| Note | The cheapest D-Luciferin potassium salt in the market. For both in vitro luciferin luciferase ATP assay and in vivo mouse imaging. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
MB102(or MB000102-R70170): D-Luciferin Potassium Salt
D-Luciferin is the small molecule substrate for the enzyme Luciferase producing bioluminescence or light. There are many types of luciferin and analogs in nature or created by chemical synthesis. The substrate and enzyme are present in a variety of light emitting organisms including fireflies and beetles. The firefly luciferin is identical to the beetle luciferin. Luciferin is also commonly used for in vitroresearch, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.
After comparing the prices of synthetic D-luciferin potassium salt from a number of suppliers, one should know where to buy D-luciferin potassium salt: Syd Labs, Inc located in Boston, USA. Syd Labs offers the best price in the worldwide to reduce researchers’ cost to purchase the chemical reagent. Its D-luciferin potassium salt price : $175 for 1 gram, $1599 for 10 g. The prices are even lower than other suppliers’ D-luciferin for sale. Syd Labs’s low price does not hurt quality of our reagent. Welcome to purchase D-luciferin potassium salt.
References of Syd Labs’s D-Luciferin Potassium Salt:
1. Cancer Immunotherapy based on Image-guided STING Activation by Nucleotide Nanocomplex-Decorated Ultrasound Microbubbles
Xuefeng Li,et al.Nat Nanotechnol.2022.PMCID: PMC9378430
“BALB/cJ mice were used to study the growth and metastasis of Luc-4T1 tumours in vivo as follows. At 12 days post tumour implantation, mice were intraperitoneally injected with 1.5 mg D-luciferin (Syd Labs) per 10 g body weight; at 10 min after injection, the presence and metastases of the tumours were monitored by bioluminescence imaging (BLI) using an In Vivo Imaging System (PerkinElmer IVIS® Lumina III).”
2. Circulating tumor cells exhibit metastatic tropism and reveal brain metastasis drivers
Remi Klotz,et al.Cancer Discov.2020.PMCID: PMC6954305
“All animal experiments were carried out in accordance with approved protocols from the Institutional Animal Care and Use Committee of USC. Metastatic tumors were established by inoculation of 1 × 105 GFP-LUC labeled CTCs in 100 μl of PBS into the left cardiac ventricles of 6–8 weeks old female NSG mice supplemented with subcutaneous slow release estrogen pills. Metastasis formation was monitored every 2 weeks by in vivo imaging using IVIS Lumina III (PerkinElmer) following intraperitoneal injection of 100 μl of D-luciferin substrate (Syd Labs).”
3. Inhibition of aberrant tissue remodelling by mesenchymal stromal cells singly coated with soft gels presenting defined chemomechanical cues
Sing Wan Wong,et al.Nat Biomed Eng.2022.PMCID: PMC8908879
“One week after bleomycin treatment, uncoated or gel-coated firefly luciferase expressing D1 mouse MSCs were delivered via i.t. or i.v. routes. The mice were then i.v. injected with 200 μl of 15 mg ml−1 D-luciferin (Syd Labs) in PBS per 20 g mouse at different time points, followed by bioluminescence imaging with the IVIS Spectrum (PerkinElmer) within 10 min of D-luciferin injection to measure an average radiance (photons s−1 cm−2 sr−1) in the lung region using the Living Image software (4.0, PerkinElmer).”
4. Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer
David Cunningham,et al.Am J Clin Exp Urol.2018.PMCID: PMC6055074
“D-luciferin potassium salt (catalogue #MB102, Syd Labs Inc., Natick, MA) was resuspended in phosphate-buffered saline (PBS) to a final concentration of 20 mg/ml. Mice were dosed with 200 μL luciferin substrate intraperitoneally and waited for 10 minutes to allow for distribution of substrate prior to image acquisition.”
5. Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer
David Cunningham, Qiuyang Zhang, Sen Liu, Keshab R Parajuli, Qiang Nie, Lin Ma, Allen Zhang, Zhenbang Chen, Zongbing You. Am J Clin Exp Urol. 2018; 6(3): 114–122.
“D-luciferin potassium salt (catalogue #MB102, Syd Labs Inc., Natick, MA) was resuspended in phosphate-buffered saline (PBS) to a final concentration of 20 mg/ml. Mice were dosed with 200 μL luciferin substrate intraperitoneally and waited for 10 minutes to allow for distribution of substrate prior to image acquisition.”
6. Cucurbitacin E inhibits the Yes?associated protein signaling pathway and suppresses brain metastasis of human non?small cell lung cancer in a murine model
Hsu PC, Tian B, Yang YL, Wang YC, Liu S, Urisman A, Yang CT, Xu Z, Jablons DM, You L. Oncol Rep. 2019 Aug;42(2):697-707.
“To monitor the condition of brain metastasis in the mice, bioluminescent imaging (Caliper Life Sciences, Waltham, MA, USA) was used. For this, mice were injected with D-luciferin potassium salt (SYD Labs, Inc., Natick, MA, USA) at a dose of 150 mg/kg i.p., anesthetized, and then placed into the Xenogen IVIS® spectrum imaging system (PerkinElmer, Boston, MA, USA) at 10 min after D-luciferin potassium salt injection.”
7. Cellular MRI Reveals Altered Brain Arrest of Genetically Engineered Metastatic Breast Cancer Cells
Parkins KM, Hamilton AM, Dubois VP, Wong SM, Foster PJ, Ronald JA. Contrast Media Mol Imaging. 2019 Jan 8;2019:6501231.
“BLI was performed using a hybrid optical/X-ray scanner (IVIS Lumina XRMS In Vivo Imaging System, PerkinElmer). Mice were anesthetized with isofluorane (2% in 100% oxygen) and received a 150 μL intraperitoneal injection of D-luciferin (30 mg/mL; Syd Labs, Inc., MA, USA), and BLI images were captured for up to 35 minutes after injection.”
D-Luciferin Potassium Salt FAQ:
Q: How to make D-luciferin stock solution and work solution:
A:To prepare solution for in vitro bioluminescent assay: Make a 200x D-luciferin stock solution (30 mg/ml) in sterile deionized water. Mix gently by inversion until the chemical is completely dissolved. Make a D-Luciferin working solution (150 ug/ml final concentration) in pre-warmed culture medium.
To prepare solution for in vivo imaging: Make a D-luciferin stock solution (15 mg/ml) in DPBS. Mix gently by inversion until the compound is completely dissolved. Filter sterilize the solution through a 0.2 um filter. Inject animals at 10 ul/g of body weight or 1.5 mg D-luciferin for a 10 g mouse.
pH stability of D-luciferin potassium salt: If pH>6.3, it may be converted to L-luciferin through a slow racemization.
Storage of D-luciferin potassium salt in powder and solution: It may form dehydroluciferin in the presence of ultraviolet light and/or moisture. The lyophilized powder should be stored in the dark at -20°C. For in vivo and in vitro use, the stock solution after reconstitution with PBS or deionized water can be stored at 4°C with protection from light for 3 weeks, and at -20°C for 3 months. Please contact us if you need protocol, datasheet, or SDS of D-luciferin potassium salt. Quality of our D-luciferin potassium salt has been proven by a number of publications citing our product.
Q: What is D-luciferin used for?:
A:Luminometers and optical microscopes have been developed to detect light emitted from the two-step luciferase-catalyzed chemical reaction. The protein Luciferase can be transiently or stably expressed in cells and animals, mostly mice. With the luciferin luciferase ATP reagent, equipment, and genetic engineering, firefly luciferase and luciferin have been widely used in in vitro and in vivo reporter assays detecting gene expression and detecting the level of cellular ATP in cell viability assays or for kinase activity assays. The luciferin luciferase ATP assay kits are commercially available. The applications of firefly luciferase and D-luciferin including in vivo imaging are still expanding.
Q: How to make D-luciferin potassium salt?:
A: Although D-luciferin is a natural compound produced in fireflies and beetles, the cost of chemical synthesis is much cheaper than extraction of the biosynthesized chemical from insects. There are D-luciferin free acid, D-luciferin potassium salt, and D-luciferin sodium salt in the market. Our synthetic D-luciferin potassium salt (catalog No. MB000102-R70170 or MB102) is purified to remove dehydroluciferin and L-luciferin, competitive inhibitors of firefly luciferase. Water solubility of the chemical is great to make stock solution with high concentration.
Q: Do you provide free sample of your D-luciferin potassium salt?
A: Sorry that we do not provide free sample. You can place an order and we will hold the invoice until you tell us that you like our product. You do not need to pay us if you do not like the product. It is an alternative to free sample.
Q: Can your D-luciferin potassium salt be injected to animals such as mice for in vivo study?
A: Yes if you filter sterilize the D-luciferin solution before animal injection. Most of our clients use D-luciferin potassium salt for in vivo imaging, as described in References above.
Q: Is D-luciferin potassium salt water soluble?
A: Yes, D-luciferin potassium salt is water soluble though its water solubility is inferior to D-luciferin sodium salt.
Q: How is pH stability of D-luciferin potassium salt?
A: Its optimal pH is 6.0 to 6.3. If pH>6.3, it may be converted to L-luciferin through a slow racemization. L-luciferin competitively inhibits the luciferase-catalyzed chemical reaction of D-luciferin producing luminescence. Because most clients use water or DPBS to dissolve D-luciferin without pH adjustment, the solution should be stored in -20C and used in no longer than 3 months.
Q: Do you ship your D-luciferin potassium salt to Europe by DHL or FedEx?
A: Yes, normally FedEx and sometimes DHL if clients request.
Q: For $1599, can you ship us 10 vials with 1 g/vial?
A: Yes.
Frequent questions related to “D-luciferin potassium salt”:
How to make D-luciferin potassium salt?
How does the luciferin luciferase system work?
How do you make luciferin stock?
How to make D-luciferin work solution?
How to make D-luciferin stock solution?
Popular searches related to “D-luciferin potassium salt“:
Luciferase reporter assay protocol
Purchase D-luciferin potassium salt
Luciferin luciferase system in vivo
Prices of synthetic D-luciferin potassium salt