Catalog No.	NC0107
Product Name	MSC Serum Free Medium (GMP Grade)
Supplier Name	YOCON
Brand Name	YOCON
Packaging Specification	Experience the convenience of our GMP-grade Serum Free Medium (SFM) for MSC, packaged in 500 ml bottles for efficient storage and use in mesenchymal stem cell culture workflows.
Storage Conditions	Store your Serum Free Medium (SFM) for MSC (GMP Grade) at 2-8°C and protect from light to ensure optimal stability and performance in MSC culture applications.
Shelf Life	Maintain a reliable 12-month shelf life for your Serum Free Medium (SFM) for MSC (GMP Grade) by adhering to proper storage guidelines, supporting long-term stem cell research efficiency.
Appearance	The Serum Free Medium (SFM) for MSC (GMP Grade) is light yellow to yellow, clear, and transparent liquid.
Osmolarity	Optimize osmotic balance in cell therapy processes with our GMP-grade Serum Free Medium (SFM) for MSC, offering an osmolarity range of 280-350 mOsm/Kg to support robust MSC growth and viability.
Endotoxin	Achieve endotoxin levels below 0.25 EU/mL in Serum Free Medium (SFM) for MSC (GMP Grade), ensuring safety and compliance for clinical-grade mesenchymal stem cell expansion.
Sterility	Ensure contamination-free results with our GMP-grade Serum Free Medium (SFM) for MSC, featuring sterility confirmed by no bacteria or fungi detected for safe mesenchymal stem cell cultivation.
Mycoplasma	Protect your stem cell cultures from mycoplasma risks using our GMP-grade Serum Free Medium (SFM) for MSC, rigorously tested and confirmed as not detected to maintain high-quality MSC expansion.
pH Value	Achieve ideal physiological conditions in your experiments with our GMP-grade Serum Free Medium (SFM) for MSC, maintaining a pH value of 6.8-7.8 at 25°C for consistent mesenchymal stem cell performance.
Note	Explore available options like Mesenchymal Stem Cells Serum-free Medium Supplement (GMP Grade) and Gentle Digestive Enzymes for Stem Cells to complement your Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0 in advanced cell therapy workflows.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

NC0107: Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0

GMP Mesenchymal Stem Cell Serum-Free Medium 2.0 is a premium, xeno-free and serum-free medium specifically designed for the clinical-scale expansion and characterization of human mesenchymal stem cells (MSCs). It uses a completely serum-free, animal-derived component-free, chemically defined formulation that meets GMP standards for production. It is suitable for the isolation, cell banking, and continuous subculture of MSCs derived from bone marrow, umbilical cord, and other tissue sources. The medium supports highly efficient cell growth while maintaining stemness, multipotency, and a completely defined composition—free from serum, animal-, or human-derived components.

By eliminating the need for serum or serum substitutes, it simplifies the culture process and enhances the efficiency and quality of stem cell culture and exosome harvesting. In short, this product helps researchers maintain high-quality cells while simplifying the process, making it an ideal choice for clinical research applications.

YOCON Serum-free MSC medium significantly improves cell adhesion and adaptability, supporting lower seeding densities and higher amplification multiples. There is no need to change the medium; one-step passaging to completion allows efficient subculture up to P20 without cell aging. With an extremely low background level of exosomes, it is compatible with 2D and 3D large-scale culture modes such as cell factories, microcarriers, and bioreactors, facilitating the harvest of higher exosome yields.

Available Formats of Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0:

Product Name	Cat. No.	Packaging Spec	Storage Conditions	Shelf Life
Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0	NC0107	500 mL/bottle	2-8℃, protect from light	12 months
Mesenchymal Stem Cells Serum-free Medium Supplement (GMP Grade)	NC0106.S	5 mL/bottle	-20℃ , protect from light	12 months
Gentle Digestive Enzymes for MSCs	NC1004.1	500 mL/bottle	2-8℃, protect from light	12 months

Key Advantages of YOCON’s Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0:

• Serum-Free & Animal-Free: Ideal for Clinical Use: All proteins are artificially recombinant, free from human or animal sources, making them suitable for clinical applications.
• Enhanced Cell Adhesion and Adaptation: Significantly improves cell attachment and adaptability, supporting lower seeding densities and higher expansion rates.
• Continuous Culture Without Medium Change: No need to change the culture medium; efficient passaging up to P20 without cell aging.
• Low Background Exosomes for Large-Scale Cultivation: Extremely low background exosome content, compatible with 2D and 3D large-scale cultivation systems like cell factories, microcarriers, and bioreactors, facilitating higher exosome yield.

Applications of YOCON’s Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0:

• Primary MSC Isolation: The GMP Grade Serum-Free MSC Culture Medium is ideal for primary isolation of mesenchymal stem cells (MSCs) from human umbilical cord and bone marrow. It ensures a serum-free environment that preserves the undifferentiated state of the cells during isolation.
• Seed Bank Establishment: This medium is highly effective for seed bank establishment, supporting long-term storage and reliable subculture of MSCs, making it a valuable tool for stem cell banking and preservation.
• Continuous Subculture of MSCs: The medium is designed to support the continuous subculture of MSCs, allowing for the maintenance of normal cell morphology and multipotential differentiation capacity without the need for serum or serum substitutes.
• Clinical Research Applications: Ideal for clinical research applications, this serum-free medium meets GMP standards, ensuring high-quality, reproducible results that are critical for regenerative medicine and stem cell therapies.
• Regenerative Medicine and Stem Cell Therapies: The product supports regenerative medicine by maintaining the multipotential differentiation capacity of MSCs, which is essential for their therapeutic use in tissue repair and regeneration.

Simplified Usage Instructions of YOCON’s Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0:

Even if users have limited experience with stem cell culture, they can easily follow these steps to culture MSCs and harvest exosomes:

• Medium Preparation: Remove the medium base (500 mL bottle) and additives (5 mL bottle) from refrigeration. Thaw the additive at 2–8°C and mix it with the medium base as per the instructions. After mixing, the complete medium is ready for use. No additional serum or growth factors are required as the medium already contains all necessary components to support MSC growth.
• Cell Seeding: Seed MSCs in culture flasks or dishes at the recommended density (approximately 8,000–10,000 cells/cm²) to achieve optimal growth. The medium is suitable for use with standard tissue culture-treated vessels without any additional coating required (Tip: Ensure the culture surface is suitable for MSC adhesion for optimal results).
• Culture Conditions: Place the flasks in a CO₂ incubator at 37°C with 5% CO₂. Change the medium after 24–48 hours to remove residual digestion enzymes and cell debris. Change the medium every 2–3 days to maintain adequate nutrient supply and remove waste products.
• Passaging & Expansion: When cells reach approximately 80–90% confluence (typically 3–4 days post-seeding), digest the cells using the provided gentle stem cell digestion enzyme . This enzyme protects cell viability better than traditional trypsin. After digestion, reseed the cells at the recommended density for the next passage. Repeat the process to expand the required number of cells.
• Exosome Harvesting: If exosome harvesting is the goal, replace the medium with fresh complete medium when cells reach the exponential growth phase (around 50–70% confluence). Continue to culture for approximately 48 hours, allowing cells to secrete sufficient exosomes. No serum adding is required—this serum-free medium supports exosome production without additional treatments. Afterward, collect the supernatant and use ultracentrifugation or other methods to isolate exosomes. It is recommended to use methods like ExtraPEG for efficient exosome enrichment .

Performance Display of YOCON’s Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0:

1. Cell passaging performance

Thawing of cryopreserved P2 cells and continuous serial subculture

Passage number	culture vessel	number of cells harvested per vessel	expansion multiple	seeding density/cm²	Days
P3	150 mm dish	1.50E+07	12.52	8000	3 days
P4	150 mm dish	1.68E+07	14.00	8000	3 days
P5	150 mm dish	1.59E+07	13.22	8000	3 days
P6	150 mm dish	1.33E+07	11.07	10000	3 days
P7	150 mm dish	1.34E+07	11.13	10000	3 days

Isolation and serial passage of primary umbilical cord MSCs

Passage number	culture vessel	number of cells harvested per vessel	expansion multiple	seeding density/cm²	Days
P0	150 mm dish	3.68E+06	0		14-16 days
P1	150 mm dish	1.50E+07	10	8000	3 days
P2	150 mm dish	1.36E+07	11.29	8000	3 days
P3	150 mm dish	1.53E+07	12.75	8000	3 days
P4	150 mm dish	1.39E+07	77.55	8000	3 days
P5	150 mm dish	1.21E+07	10.08	8000	3 days
P6	150 mm dish	1.54E+07	10.28	10000	3 days
P7	150 mm dish	1.75E+07	11.64	10000	3 days
P8	150 mm dish	1.50E+07	10.00	10000	3 days
P9	150 mm dish	1.73E+07	11.56	10000	3 days
P10	150 mm dish	1.45E+07	9.64	10000	3 days

2. Exosome culture performance

2.1 This product is used to produce exosomes in cell factories.
P2 cells were revived and continuously passaged to P3 cells before being inoculated into cell factories. A total of 2.52E+12 exosomes were harvested in 15 days.

Calculation of total amount of harvested exosomes

Sampling time point	Exosome concentration (pieces/mL)	Number of exosomes harvested (pieces)	Culture medium volume (mL)
Day 3	3.53E+09	4.24E+11	120
Day 6	5.36E+09	6.43E+11	120
Day 9	4.76E+09	5.71E+11	120
Day 12	5.46E+09	6.55E+11	120
Day 15	1.91E+09	2.29E+11	120
Total.		2.52E+12	600

2.2 This product is used to harvest exosomes from microcarrier 3D cultured cells
The cycle is 15 days, 500mL of culture medium, and a total of 3.50E+12 exosomes were harvested.

Calculation of total amount of harvested exosomes

Sampling time point	Exosome concentration (pieces/mL)	Number of exosomes harvested (pieces)	Culture medium volume (mL)
Day 3	6.19E+09	6.19E+11	100
Day 6	9.26E+09	9.26E+11	100
Day 9	7.92E+09	7.92E+11	100
Day 12	6.06E+09	6.06E+11	100
Day 15	5.54E+09	5.54E+11	100
Total.		3.50E+12	500

YOCON provides the following related products of Mesenchymal Stem Cell (MSC) Serum-free Medium (GMP Grade):

Mesenchymal Stem Cells Serum-free Medium Supplement (GMP Grade)

Gentle Digestive Enzymes For Stem Cells

YOCON provides the following Mesenchymal Stem Cell (MSC) Serum-free Medium:

Mesenchymal Stem Cells Serum-free Medium

Mesenchymal Stem Cells Serum-free Medium (GMP Grade)

The Serum Free Medium (SFM) for MSC (GMP Grade) 2.0 from: Serum Free Medium (SFM) for MSC (GMP Grade) 2.0 NC0107 YOCON

No more offers for this product!