Monoclonal Antibody Immunoconjugation Service
We will link two approved anti-tumor antibiotics to your purified monoclonal antibody using our proprietary conjugation method. The anti-tumor agent will remain bound to the monoclonal antibody and inactive but will be released and activated by reduction once the immunocomplex binds to the cell surface antigen and internalized.
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| Catalog No. | BS039 |
|---|---|
| Product Name | Monoclonal Antibody Immunoconjugation Service |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Summary | We will link two approved anti-tumor antibiotics to your purified monoclonal antibody using our proprietary conjugation method. The anti-tumor agent will remain bound to the monoclonal antibody and inactive but will be released and activated by reduction once the immunocomplex binds to the cell surface antigen and internalized. |
| Order Offline | Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card. |
Description
1. Pilot Study
Material required
3~5 mg purified monoclonal antibody needed
Phase I Generation of antibody immunoconjugates
? Exchange the antibody buffer to the conjugation buffer
? Activation of the antibody
? Conjugation of the antibody to TWO different drugs at TWO different molar ratio
? Purification of the immunoconjugates
? Filter-sterilization
Phase II In vitro Bioassay
? Seeding the cells in 96-well plate, up to THREE different cell lines
? Adding the immunoconjugates at various concentrations.
? Performing MTT assay on day 5.
? Repeat the bioassay twice
2. Medium-size Production
Material required
5~10 mg purified monoclonal antibody needed
Phase I Generation of antibody immunoconjugates
? Exchange the antibody buffer to the conjugation buffer
? Activation of the antibody
? Conjugation of the antibody to ONE drug at TWO different molar ratio
? Purification of the immunoconjugates
? Filter-sterilization
Phase II In vitro Bioassay
? Seeding the cells in 96-well plate, up to THREE different cell lines
? Adding the immunoconjugates at various conc.
? Performing MTT assay on day 5.
? Repeat the bioassay once
? Sending out 2 mg immunoconjugate.
Phase III In vivo animal study
? Innoculate 30 nude mice with the right number of tumor cells
? Once the tumors reach 10 mm, 5 mice per group will be injected with various amount of immunoconjugate, naked antibody, unconjugated drug and buffer
? Tumor size will be measured every 3 or 4 days
? At the end of the test, the tumors will be removed and weighed individually.
? Repeat the animal study twice.
Additional information
| Ship from Country | USA |
|---|---|
| Size | 1 package |