[KO Validated] MLH1 Mouse mAb (A21882)

Catalog No.	A21882
Product Name	[KO Validated] MLH1 Mouse mAb (A21882)
Supplier Name	ABclonal, Inc.
Brand Name	Abclonal
Synonyms	FCC2; COCA2; HNPCC; MLH-1; hMLH1; HNPCC2; LYNCH2; MMRCS1
Gene Name	MLH1
Protein Name	MLH1
Uniprot/Swissprot ID	P40692
Entrez GeneID	4292
Clone	AMC0417
Clonality	Monoclonal
Source/Host	Mouse
Applications	WB, IHC-P
Reactivity	Human
Conjugate	Unconjugated
Note	Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com.
Order Offline	Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com

Description

A21882: [KO Validated] MLH1 Mouse mAb

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).

Immunogen Information about [KO Validated] MLH1 Mouse mAb (A21882)

Immunogen：Recombinant protein of human MLH1.
Sequence：service@abclonal.com
Gene ID：4292
Swiss prot：P40692
Synonyms：FCC2; COCA2; HNPCC; MLH-1; hMLH1; HNPCC2; LYNCH2; MMRCS1; H1
Calculated MW：85kDa
Observed MW：Refer to figures

Images of [KO Validated] MLH1 Mouse mAb (A21882)

Western blot analysis of lysates from Jurkat cells, using [KO Validated] MLH1 Mouse mAb (A21882) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from wild type (WT) and MLH1 Mouse mAb knockout (KO) HeLa cells, using [KO Validated] MLH1 Mouse mAb (A21882) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Please remember our product information: [KO Validated] MLH1 Mouse mAb (Catalog Number: A21882) Abclonal

Additional information

Ship from Country	USA
Size	20 ul, 100 ul, 200 ul, 50 ul

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