HiScript III One Step qRT-PCR Probe 5 × Master Mix Q611-EN

Description

Q611-EN:HiScript III One Step qRT-PCR Probe 5 × Master Mix

Product Introduction

HiScript III One Step qRT-PCR Probe 5 × Master Mix is specially designed for single-plex or multiplex qPCR detection using RNA (e.g. virus RNA) as template. It has extremely high stability, which can be stored at 37℃ for 14 days and its performance remains unchanged, and remains liquid form at a storage temperature of – 20℃ to avoid the thawing process of reagents. The reaction can be started by directly adding the template, primers and target probes required for detection. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe 5 × Master Mix can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).
Product Features
1. Using gene specific primers (GSP), the reverse transcription and
qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination
2. More sensitive amplification that is suitable for DNA and RNA templates
3. More stable amplification curve with low concentration template
4. Better storage conditions
5. It supports rapid procedures and is suitable for rapid reactions
Storage
Store at -30 ~ -15℃ and transport at ≤0℃.

Please remember our product information: HiScript III One Step qRT-PCR Probe 5 × Master Mix catalog number: Q611-EN Vazyme.

Additional information

Ship from Country	USA
Size	100 rxns, 1000 rxns, 10000 rxns

No more offers for this product!