Catalog No.	NC1004
Product Name	Gentle Digestive Enzymes For Stem Cells
Supplier Name	YOCON
Brand Name	YOCON
Packaging Specification	500 mL/bottle, 100 mL/bottle
Storage Conditions	Store your GMP-grade Gentle Digestive Enzymes for Stem Cells at 2-8°C and protect from light to ensure optimal stability and performance in MSC culture applications.
Shelf Life	Maintain a reliable 12-month shelf life for your GMP-grade Gentle Digestive Enzymes for Stem Cells by adhering to proper storage guidelines, supporting long-term stem cell research efficiency.
Endotoxin	Achieve endotoxin levels below 0.25 EU/mL in GMP-grade Gentle Digestive Enzymes for Stem Cells, ensuring safety and compliance for clinical-grade mesenchymal stem cell expansion.
Note	Explore available options like Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0 and Mesenchymal Stem Cells Serum-free Medium Supplement (GMP Grade) to complement your Gentle Digestive Enzymes for Stem Cells in advanced cell therapy workflows.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

NC1004: Gentle Digestive Enzymes For Stem Cells

The Gentle Digestive Enzymes For Stem Cells are animal-derived free digestive enzymes, a high-performance Trypsin alternative specifically developed for the passaging and dissociation of mesenchymal stem cells (MSCs). Compared with conventional trypsin, it is gentler and less harmful, helping you achieve more consistent control over digestion time and strength while maintaining robust cell quality, ensuring the cell viability of stem cells above 90% after 15 minutes of infiltration in digestive enzymes. After digestion, only dilution of culture supernatant is required without termination, so this product is better suitable for large-scale cell production. This product is free of any animal-derived components with defined components, greatly reducing the workload and difficulty in declaration of stem cell drugs.

Available Formats of Gentle Digestive Enzymes For Stem Cells:

Product name	Item No.	Product specification	Purpose
Gentle digestive enzymes for stem cells	NC1004.1	500 mL/bottle	Specially designed for digestion and passage of mesenchymal stem cells
	NC1004.2	100 mL/bottle

Key Benefits of YOCON’s Gentle Digestive Enzymes For Stem Cells:

• Free of serum and animal-derived components: This product is an animal-derived gene-free recombinant digestive enzymes, without residual exogenous protein and virus. Artificial recombinant system, without human or animal derived components, and the endotoxin in the products can be controlled at <0.25EU/mL. It has been filed with the FDA DMF, with the filing No. of 37790, which greatly reduces the workload and difficulty in submitting the application for stem cell drugs.
• Gentle digestion: Normal digestion lasts for 1-2 minutes, easy to control. High operational fault tolerance, suitable for large-scale production.
• Higher protection for cells: During normal digestion, the cell viability is 10% higher than that of conventional pancreatic enzymes, and the cell recovery is 11%-14% higher than that of conventional pancreatic enzymes.
• Extreme digestion lasts for up to15 minutes: Extreme digestion of cells lasts for up to 15 minutes almost without any effect, and cells still grow normally after subculture.

New-Generation Digestion Product:

Comprehensive comparison of the new-generation digestion products (stem cell mild digestive enzyme) and traditional digestion product (porcine and bovine natural trypsin’s)

Category	Subcategory/Question	Traditional Product	Stem Cell Mild Digestive Enzyme
Digestion performance	Normal digestion (without excessive digestion) – Cell viability	83%	above 95%
Digestion performance	Normal digestion (without excessive digestion) – Cell count after culture	1.86×10^6	1.94×10^6
Digestion performance	Digest overtime (excessive digestion) – Cell viability	60%	above 95%
Digestion performance	Digest overtime (excessive digestion) – Cell count after culture	1.07×10^6	1.88×10^6
Digestion performance	Extreme digestion (15-minute digestion) – Cell viability	0-10%	above 90%
Digestion performance	Extreme digestion (15-minute digestion) – Cell count after culture	Unable to harvest cells	1.78×10^6
Operation convenience	Does it need to be terminated after digestion?	Required	No
Operation convenience	Does it need to be stored at -20°C?	Required	No
Operation convenience	Is it ready-for-use?	No	Yes
Operation convenience	Cost of digestion termination	High. Serum, complete media or trypsin inhibitors are required	Digestion does not need to be terminated. It can be used by being diluted with the culture supernatant
Operation convenience	Cost of virus test before cell application	High. Animal viruses such as swine foot-and-mouth disease and bovine mad cow disease need to be tested	No test is required since there is no animal source
Product safety	Is it free of animal-derived components?	No	Yes
Product safety	Is it possible to confirm that there is no animal virus?	No	Yes
Difficulty in the application of cell drugs	Confirmed difficulty affected by product residues	Extremely high. Impurity components are unknown and difficult to prove	Low. The ingredients are clear, all of which are artificially synthesized components

*The above data are the corresponding data on P4 cells obtained passaging P3 cells harvested after digesting the P2 seed cells inoculated with umbilical cord-derived MSCs in T25 culture fla sks at the seeding density of 8000 cells/cm2 after 72 hours.

Compared With Traditional Trypsin, Digestive Enzymes Perform Better:

P2 MSC seed cells were subcultured for 72 hours and then digested with mild enzyme and traditional trypsin, respectively, to obtain P3 MSCs, and the cell viability after digestion was detected.

P3 MSC cells were further cultured for 72 hours, digested with mild enzyme and traditional trypsin, respectively, to harvest P4 MSC cells, and the cells were counted.

Type	Traditional trypsin	Mild digestive enzyme
P3 MSC cell Viability	85%	95%
P3 MSC cell count harvested	2.06×106 cells	2.28×106 cells
P4 MSC cell Viability	83%	96%
P4 MSC cell count harvested	2.06×106 cells	1.54×106 cells

 

Item	Sub-item	Conventional product	Gentle digestive enzyme for stem cells
Digestive performance	Proper digestion (Without) – Cell viability	83%	above 95%
Digestive performance	Proper digestion (Without) – Cells obtained by continuous culture after digestion	1.86×10^6	1.94×10^6
Digestive performance	Overtime digestion (With over-digestion) – Cell viability	60%	above 95%
Digestive performance	Overtime digestion (With over-digestion) – Cells obtained by continuous culture after digestion	1.07×10^6	1.88×10^6
Digestive performance	Extreme digestion (15 min digestion) – Cell viability	0-10%	above 90%
Digestive performance	Extreme digestion (15 min digestion) – Cells obtained by continuous culture after digestion	None	1.78×10^6
Convenience	Termination required after digestion?	Yes	No
Convenience	Kept at -20°C?	Yes	No
Convenience	Ready for pickup and use?	No	Yes
Comprehensive cost of use	Cost of digestion termination	High. Serum, complete media, or trypsin inhibitors are required.	None. No digestion termination is required. It is enough to dilute with culture supernatant.
Comprehensive cost of use	Cost of virus test before cell application	High. Animal viruses such as swine foot-and-mouth disease and bovine mad cow disease need to be tested.	None. No test is required as it is free of animal-derived components.
Product safety	Free of animal-derived components?	No	Yes
Product safety	Confirm the absence of animal viruses?	No	Yes
Difficulty in declaration of cell drugs	Difficulty in demonstration of the effect of residual product	Extremely large. It is difficult to demonstrate because impurity components are unknown.	Low. The ingredients are clear, all of which are artificially synthesized components.

* The above data was the corresponding data obtained from P4 cells obtained by inoculating P2 seed cells of MSC of umbilical cord origin in T25 culture flasks with an inoculation density of 8,000 cells/cm² and subculturing the P3 cells obtained by digestion after 72 h.

YOCON provides the following related products of Mesenchymal Stem Cell (MSC) Serum-free Medium (GMP Grade):

Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0

Mesenchymal Stem Cells Serum-free Medium Supplement (GMP Grade)

The Gentle Digestive Enzymes For Stem Cells from: Gentle Digestive Enzymes For Stem Cells: NC1004 YOCON

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