Cell and Tissue Direct PCR Kit
$109.00
Syd Labs Cell and Tissue Direct PCR Kit MB103-11, Cell and Tissue Direct PCR Kits can be used for both easy and rapid genomic DNA isolation and preparation from animal cells and tissue samples coupled with PCR.
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Catalog No. | MB103-11 |
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Product Name | Cell and Tissue Direct PCR Kit |
Supplier Name | Syd Labs, Inc. |
Brand Name | Syd Labs |
Note | The cheapest Cell and Tissue Direct PCR Kits and PCR Master Mixes in the market. Free samples of Cell and Tissue Direct PCR Kits and PCR Master Mixes may be available. |
Order Offline | Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card. |
Description
MB103-11: Cell and Tissue Direct PCR Kit
Background
Cell and Tissue Direct PCR Kit = Rapid Cell and Tissue Genomic DNA Extraction Kit (Catalog No. MB103-10) + 2x PCR PreMix, with dye (Catalog No. MB067-EQ2-100). The kits and reagents are routinely used in mutation detection, SNP genotyping, and target detection in transgenic animals.
Rapid genomic DNA extraction:
Step 1:
A) Mouse tails: Place 2-3 mm mouse tail in a 0.5 ml microcentrifuge tube containing 100 ul solution A.
B) Cultured cells: Add 1×10^4 – 1×10^5 cell suspension in <10 ul to a 0.5 ml microcentrifuge tube containing 100 solution A or add cell pellet that contains 1x10^4 - 1x10^5 cells. Step 2:
Place the tube in a thermocycler and heat at 95°C for 5 min. Cool the tube to room temperature. Add 10 ul solution B and vortex briefly. Extracted genomic DNA is now ready for use as PCR temperate or for longer term storage at -20°C.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
Please remember our product information: Cell and Tissue Direct PCR Kit: MB103-11 Syd Labs
Additional information
Ship from Country | USA |
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Size | 100 reactions |