CD13/ANPEP Rabbit mAb (A21268)
$148.00 – $548.00
Abclonal CD13/ANPEP Rabbit mAb (Catalog Number: A21268) Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes.
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| Catalog No. | A21268 |
|---|---|
| Product Name | CD13/ANPEP Rabbit mAb (A21268) |
| Supplier Name | ABclonal, Inc. |
| Brand Name | Abclonal |
| Synonyms | APN; AP-M; AP-N; CD13; LAP1; P150; PEPN; hAPN; GP150 |
| Gene Name | ANPEP |
| Protein Name | ANPEP |
| Uniprot/Swissprot ID | P15144 |
| Entrez GeneID | 290 |
| Clone | ARC53706 |
| Clonality | Monoclonal |
| Source/Host | Rabbit |
| Applications | WB, IHC-P, FC |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Note | Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com. |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
A21268: CD13/ANPEP Rabbit mAb
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma.
Immunogen Information about CD13/ANPEP Rabbit mAb (A21268)
Immunogen：Recombinant fusion protein containing a sequence corresponding to amino acids 69-967 of human CD13/ANPEP (NP_001141.2).
Sequence：KAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK
Gene ID：290
Swiss prot：P15144
Synonyms：APN; AP-M; AP-N; CD13; LAP1; P150; PEPN; hAPN; GP150; CD13/ANPEP
Calculated MW：110kDa
Observed MW：160kDa
Images of CD13/ANPEP Rabbit mAb (A21268)
Western blot analysis of various lysates using CD13/ANPEP Rabbit mAb (A21268) at1:40000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded human breast cancer using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded human breast using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded Human cervical squamous cell carcinoma using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded human colon carcinoma using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded human endometrium cancer using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded human extranodal NK-T cell lymphoma using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded human placenta using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of CD13/ANPEP in paraffin-embedded Human endometrial stromal sarcoma using CD13/ANPEP Rabbit mAb (A21268) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow cytometry:1X10^6 Jurkat cells (negative control, left) and HepG2 cells (right) were surface-stained with CD13/ANPEP Rabbit mAb(A21268, 2.5 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 10 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-mouse pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Please remember our product information: CD13/ANPEP Rabbit mAb (Catalog Number: A21268) Abclonal
Additional information
| Ship from Country | USA |
|---|---|
| Size | 20 ul, 100 ul, 200 ul, 50 ul |