Catalog No.	PA007461
Product Name	Anti Human HLA ABC Antibody, clone W6/32 | PA007461
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA), HLA class I histocompatibility antigen
Clone	W6/32.
Isotype	Mouse IgG2a Kappa.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: W6/32) specifically binds to mouse HLA-ABC.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse HLA-ABC protein.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The in vivo grade recombinant anti-human HLA-ABC antibodies (clone of W6/32) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007461: Recombinant Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32), Mouse IgG2a Kappa, In vivo Grade

The in vivo grade recombinant anti-human HLA-ABC Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells. The anti-human HLA-ABC antibody is a monoclonal or polyclonal antibody that targets conserved, non-polymorphic epitopes shared across human Major Histocompatibility Complex (MHC) Class I molecules (HLA-A, HLA-B, and HLA-C), which are expressed on the surface of nearly all nucleated cells. This antibody recognizes common regions of the HLA-ABC complex and is widely employed in immunological research for techniques such as flow cytometry, Western blotting, and immunohistochemistry to detect cell surface markers, investigate immune responses, and study transplantation-related mechanisms. HLA-ABC proteins play a critical role in immune surveillance and cell recognition, and the anti-human HLA-ABC antibody—particularly well-established clones like W6/32—has become a reliable and broadly used tool in the field due to its pan-specific reactivi

References of Anti-Human HLA-ABC Monoclonal Antibody:

1、HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging FcγRs.
Valenzuela, N. M., et al. J Immunol. 2013 Jun 15;190(12):6635-50. doi: 10.4049/jimmunol.1201434. PMID: 23690477
“We investigated the mechanisms underlying monocyte recruitment by HLA class I (HLA I) Ab–activated endothelium. …We used a panel of murine mAbs of different subclasses to crosslink HLA I on human aortic, venous, and microvascular endothelial cells and measured the binding of human monocytic cell lines and peripheral blood monocytes. …Accordingly, HLA I mIgG2a but not mIgG1 treatment of endothelial cells significantly augmented recruitment, predominantly through FcγRI, and, to a lesser extent, FcγRIIa. …We confirmed these observations using human HLA allele-specific mAbs and IgG purified from transplant patient sera. …Importantly, the subclass of donor-specific Ab may influence its pathogenesis.”

2、Blockade of p-selectin is sufficient to reduce MHC I antibody-elicited monocyte recruitment in vitro and in vivo.
Hong, L., et al. Am J Transplant. 2013 Feb;13(2):299-311. doi: 10.1111/ajt.12016. PMID: 23279566
“Endothelial vesicles known as Weibel-Palade bodies (WPb) are mobilized after HLA I crosslinking, leading to adherence of the immature neutrophilic cell line HL60. …In addition, macrophage infiltration frequently accompanies antibody-mediated rejection (AMR), and correlates with donor specific HLA antibodies and poor graft outcome. …It is incompletely understood how HLA I antibodies may promote monocyte recruitment into the allograft. …This study demonstrates that selectin blockade prevents monocyte adherence in vitro and intragraft macrophage accumulation in vivo in response to MHC I antibodies. …The use of the human aortic tissue for the research described herein was approved by the OneLegacy Biomedical Review Board under the agreement #RS-02-10-2 and UCLA MTA2009-561.”

For more references about Anti-Human HLA-ABC Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
Recombinant mouse IgG2a isotype control antibody, In vivo grade

Anti-human HLA-ABC (W6/32) from: Anti-Human HLA-ABC Monoclonal Antibody, Mouse IgG2a Kappa (Clone: W6/32): PA007461 Syd Labs

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