Catalog No.	PA007706.h1LA
Product Name	Anti-Biotin Monoclonal Antibody (Clone: M33) | PA007706.h1LA
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Biotin; Vitamin H; Vitamin B7
Clone	M33.
Isotype	Human IgG1-L234A L235A P329G (LALAPG) Kappa
Source/Host	The recombinant anti-Biotin monoclonal antibody (clone: M33) was produced in mammalian cells.
Specificity/Sensitivity	The recombinant anti-Biotin monoclonal antibody (clone: M33) specifically binds to Biotin.
Applications	ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)).
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The recombinant anti-Biotin monoclonal antibody of clone M33 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant Human IgG1-L234A L235A P329G (LALAPG) isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007706.h1LA: Recombinant Anti-Biotin Monoclonal Antibody (Clone: M33), Human IgG1-L234A L235A P329G (LALAPG) Kappa

Recombinant Human IgG1-L234A L235A P329G (LALAPG) Monoclonal Antibody.

Background of Recombinant Anti-Biotin Monoclonal Antibody

Recombinant mouse anti-biotin antibodies recognize biotin (vitamin B7) attached to proteins, peptides, nucleic acids, or solid matrices, and can be offered in fluorophore and enzyme-conjugated versions.

The anti-biotin monoclonal antibody can be used as a second-step reagent in various applications for detecting biotinylated targets. The biotin-streptavidin system is widely used in immunoassay signal amplification techniques because streptavidin binds biotin with extremely high affinity.

Because biotin is naturally present in a large variety of biological samples, it may affect the results of biotin-based assays, such as reduced sensitivity, false negatives or false positives. Streptavidin/biotin blocking kit, avidin/biotin blocking system, or biotin blockers are used to block signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection.

Please contact us to ask for a quote for the engineered anti-biotin antibody mutants of various isotypes, and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated antibodies and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-biotin antibodies and mutants are available.

References of Anti-Biotin Monoclonal Antibody:

Improved elution strategy and new monoclonal anti-biotin antibody for LC-MS/MS characterization of protein biotinylation sites
Yiying Zhu, et al. Biochem Biophys Rep.2024 Apr 18:38:101711. doi: 10.1016/j.bbrep.2024.101711. PMID: 38681669
In this study, we made a crucial improvement to the elution phase of the immunoaffinity enrichment step for biotinylated peptides, which involves the addition of a highly organic solvent, and developed a monoclonal anti-biotin antibody that improved the identification number for biotinylated peptides. In this study, we developed a monoclonal anti-biotin antibody to ensure the reproducibility and performance of the method. The approach involved developing a monoclonal anti-biotin antibody with better reproducibility as compared to existing polyclonal antibodies (pAbs). Samples were incubated with anti-biotin antibody beads for 1 h at 4 °C. We further compared the results from our anti-biotin antibody enrichment method and those from Paek et al.
Tags: activity of Anti-Biotin Monoclonal Antibody; activity of Anti-Biotin Monoclonal Antibody antibody

Development of an ultrasensitive sandwich immunoassay for detecting small molecule semicarbazide
Shiwei Zhang, et al. Food Chem.2023 Dec 15:429:136835. doi: 10.1016/j.foodchem.2023.136835. PMID: 37463535
Sandwich ELISA for SEM derivatives was developed using an anti-SEM-NBA antibody and horseradish peroxidase-labeled avidin or anti-biotin antibody as a secondary conjugate. Anti-biotin antibody-HRP and avidin-HRP were purchased from Sigma-Aldrich (St Louis, MO, USA), biotin (S)-sulfoxide and lidimycin were purchased from TopScience (Shanghai, China), and 96-well ELISA plates were purchased from Corning (USA). The advantageous distance and optimal distance between epitopes under these two modes were also calculated. The failure to develop sandwich ELISA for small molecules is not directly caused by their molecular weight but largely by the steric hindrance caused by the small distance between epitopes. The obtained optimal EC50 values were 11.2 pg/mL (double-antibody sandwich with the epitope distance ≥16 Å) and 7.3 pg/mL (antibody-avidin sandwich with the epitope distance ≥17 Å).
Tags: Anti-Biotin Monoclonal Antibody antibody; Anti-Biotin Monoclonal Antibody antibody for animal model

Development of a novel flow cytometry method for detecting pneumococcal-specific B cells
Irene Tzovara, et al. Cytometry A.2022 Jul;101(7):588-596. doi: 10.1002/cyto.a.24654. PMID: 35527678
PS was chemically biotinylated and mounted on anti-biotin beads, and labeled with phycoerythrin (PE)-conjugated anti-biotin antibody to form a PS-multimer used for cell staining. bPS-beads were washed once and then resuspended in 100 μl of washing buffer before labeling with anti-biotin-PE. Excess anti-biotin-PE was removed by washing the labeled bPS-beads three times. The ratio of bPS-beads to anti-biotin-PE was tested in a stepwise manner at v/v ratios of: 50:0.5, 50:1, 50:2, 50:4, and 50:8 for each pneumococcal serotype. A negative control tube containing anti-biotin-PE but no bPS monomer was used for each blood sample.
Tags: Anti-Biotin Monoclonal Antibody antibody for cancer research; Anti-Biotin Monoclonal Antibody antibody for mouse tumor model

Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification
Quynh T Phan, et al. Methods Mol Biol.2021:2260:27-36. doi: 10.1007/978-1-0716-1182-1_3. PMID: 33405029
Receptors on endothelial and epithelial cells often recognize molecules that are expressed by fungi, and only a limited number of these receptors have been identified to date. Here, we describe a method for identifying novel host cell receptors for fungi that uses intact organisms to precipitate biotin-labelled host cell membrane proteins, which are then detected by immunoblotting with an anti-biotin antibody. Presented here is the method to use for identification of membrane proteins that bind to C. albicans.
Tags: Anti-Biotin Monoclonal Antibody antibody in vivo; Anti-Biotin Monoclonal Antibody for animal model

Development of Reverse Transcriptase Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Tilapia Lake Virus (TiLV): Pilot Study
Saralee Srivorakul, et al. Vet Sci. 2025 Sep 1;12(9):845. doi: 10.3390/vetsci12090845. PMID: 41012770
When the complex reached the nitrocellulose membrane, the test line, coated with the immobilized anti-Biotin capture antibody, was captured with the Biotin tag on the other end of the amplicon. To adapt the Basic Kit for LFD, primers were modified with a 5′-FITC label and a 5′-Biotin tag, enabling amplicon capture and visualization on the dipstick via anti-FITC and anti-Biotin antibodies. The test was generated by specific anti-FITC and anti-Biotin capture antibodies that are compatible with the TiLV-specific primers tagged with FITC and Biotin. In this conjugate pad, where the gold nanoparticles (AuNPs) were coated, the AuNPs-anti-FITC probe was applied to bind with the FITC tag on each amplicon via antibody–antigen interactions, resulting in AuNPs-anti-FITC–DNA complexes that continued to migrate along the strip. When the complex reached the nitrocellulose membrane, the test line, coated with the immobilized anti-Biotin capture antibody, was captured with the Biotin tag on the other end of the amplicon.
Tags: Anti-Biotin Monoclonal Antibody for mouse tumor model; function of Anti-Biotin Monoclonal Antibody antibody

For more references about Anti-Biotin Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-Biotin antibodies:

In Vivo Grade Recombinant Anti-Biotin Antibodies (Clone F1)

Syd Labs provides the following recombinant anti-Fluorescein/Digoxin/Biotin/Phospho-Tyrosine monoclonal antibodies:

Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4-4-20 (enhanced))
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4M5.3)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 5A7)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 26-10)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone M33)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone Py20)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone 4G10)

Recombinant Anti-Biotin Monoclonal Antibody (Clone: M33), Human IgG1-L234A L235A P329G (LALAPG) Kappa from: Recombinant Anti-Biotin Monoclonal Antibody (Clone: M33), Human IgG1-L234A L235A P329G (LALAPG) Kappa: PA007706.h1LA Syd Labs

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