Red Blood Cell Lysis Buffer (10x)
$60.00
Red Blood Cell Lysis Buffer (10x)
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| Catalog No. | 310115 |
|---|---|
| Product Name | Red Blood Cell Lysis Buffer (10x) |
| Supplier Name | Caprico Biotechnologies, Inc. |
| Brand Name | Caprico |
| Stability & Storage | 2-8oC |
| Note | Products will be shipped from the warehouse in Georgia. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com. |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
7‐Aminoactinomycin D (7‐AAD) is a DNA binding dye which can only penetrate dead or damaged cells, while it is excluded from cells having an intact membrane. Thus, it is used to include only alive cells (7-AAD negative) into Flow cytometry data analysis. 5μl of this product is sufficient for staining of 1 million cells in a 100μl staining volume or 100μl of whole blood. Add 5ul to a sample of antibody stained cells, incubate for 5 minutes on ice (protect from light) and run samples in Flow cytometer instrument. Discrimination between viable and non-viable cells is carried out through gating in the appropriate Fluorescence channel (Ex./Em. 550nm/648nm). To achieve reliable results, incubation of cells with 7-AAD for a prolonged time (>60 minutes) should be avoided.