Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1) | PA000274.mf

Catalog No.	PA000274.mf
Product Name	Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Binds to the same epitope as Sigma 'Anti-FLAG' M2 Antibody
Summary	The Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1) is a chimeric mouse/human monoclonal antibody produced in mammalian cells. It consists of mouse variable regions and human IgG1 kappa constant regions.
Clone	L1.1
Specificity/Sensitivity	detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins.
Applications	ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Reactivity	Human, Mouse, Rat, Others.
Purification	The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form Of Antibody	1 mg/ml in 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. 1 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Note	In vivo grade recombinant DYKDDDDK-Tag monoclonal antibodies with various isotypes; alternative of the monoclonal FLAG M2 antibody; for in vitro and in vivo studies including protein purification; low prices for bulk order. FLAG is a registered trade mark of Sigma Aldrich, and used here for informational purposes only.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA000274.mf: Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1), In vivo Grade

References of Anti-DYKDDDDK-Tag Antibody (clone L1.1)

1. Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme
Weiwei Peng, et al.J Proteome Res. 2021 Jul 2;20(7):3559-3566. doi: 10.1021/acs.jproteome.1c00169. PMID: 34121409; PMCID: PMC8256418
“Antibody sequence information is crucial to understanding the structural basis for antigen binding and enables the use of antibodies as therapeutics and research tools. Here, we demonstrate a method for direct de novo sequencing of monoclonal IgG from the purified antibody products. The method uses a panel of multiple complementary proteases to generate suitable peptides for de novo sequencing by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a bottom-up fashion.”

2. DECODE enables high-throughput mapping of antibody epitopes at single amino acid resolution
Katsuhiko Matsumoto, et al.PLoS Biol. 2025 Jan 23;23(1):e3002707. doi: 10.1371/journal.pbio.3002707. PMID: 39847587; PMCID: PMC11756784
“Antibodies are extensively used in biomedical research, clinical fields, and disease treatment. However, to enhance the reproducibility and reliability of antibody-based experiments, it is crucial to have a detailed understanding of the antibody’s target specificity and epitope. In this study, we developed a high-throughput and precise epitope analysis method, DECODE (Decoding Epitope Composition by Optimized-mRNA-display, Data analysis, and Expression sequencing).”

3. Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition
Xin-Yu Guo, et al.PLoS One. 2021 May 5;16(5):e0250805. doi: 10.1371/journal.pone.0250805. PMID: 33951064; PMCID: PMC8099120
“A FLAG tag consisting of DYKDDDDK is an epitope tag that is frequently and widely used to detect recombinant proteins of interest. In this study, we performed a CRISPR-based genetic screening to identify factors involved in the detection of a FLAG-tagged misfolded model protein at the cell surface. In the screening, SLC35B2, which encodes 3′-phosphoadenosine-5′-phosphosulfate transporter 1, was identified as a necessary component.”

4. Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures
Takushi Miyoshi, et al.Cell Rep. 2021 Feb 2;34(5):108708. doi: 10.1016/j.celrep.2021.108708. PMID: 33535030; PMCID: PMC7904085
“Fast-dissociating, specific antibodies are single-molecule imaging probes that transiently interact with their targets and are used in biological applications including image reconstruction by integrating exchangeable single-molecule localization (IRIS), a multiplexable super-resolution microscopy technique. Here, we introduce a semi-automated screen based on single-molecule total internal reflection fluorescence (TIRF) microscopy of antibody-antigen binding, which allows for identification of fast-dissociating monoclonal antibodies directly from thousands of hybridoma cultures. We develop monoclonal antibodies against three epitope tags (FLAG-tag, S-tag, and V5-tag) and two F-actin crosslinking proteins (plastin and espin).”

5. A perspective toward mass spectrometry-based de novo sequencing of endogenous antibodies
Sebastiaan C de Graaf, et al.MAbs. 2022 Jan-Dec;14(1):2079449. doi: 10.1080/19420862.2022.2079449. PMID: 35699511; PMCID: PMC9225641
“A key step in therapeutic and endogenous humoral antibody characterization is identifying the amino acid sequence. So far, this task has been mainly tackled through sequencing of B-cell receptor (BCR) repertoires at the nucleotide level. Mass spectrometry (MS) has emerged as an alternative tool for obtaining sequence information directly at the – most relevant – protein level.”

For more technical references or data sheets regarding the Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1) formats, please contact our scientific support team at message@sydlabs.com.

Other DYKDDDDK Antibodies:
DYKDDDDK-tag Monoclonal Antibody
DYKDDDDK-tag Monoclonal Antibody (Magnetic Microbead-Conjugated)
DYKDDDDK-tag Monoclonal Antibody (Agarose-Conjugated)

DYKDDDDK Peptide:
DYKDDDDK Peptide

Other Tag Antibodies:
HA-Tag Monoclonal Antibody (Clone 12CA5)
His-Tag Monoclonal Antibody (Clone 3D5)
Myc-Tag Monoclonal Antibody (Clone 9E10)
His-tag Monoclonal Antibody (Clone HIS.H8)
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
His-Tag Monoclonal Antibody (Clone 26D11)
V5-tag Monoclonal Antibody (Clone V5.E3)

Loading Control Antibodies:
GAPDH Monoclonal Antibody
beta-Actin Monoclonal Antibody
beta-Tubulin Monoclonal Antibody

Anti-DYKDDDDK-Tag Antibody (L1.1) from: Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1): PA000274.mf Syd Labs