D-Luciferin Potassium Salt
$175.00 – $1,599.00
The cheapest D-Luciferin potassium salt in the market. For both in vitro luciferin luciferase ATP assay and in vivo mouse imaging.
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Description
MB102: D-Luciferin, Potassium Salt
CAS No.: 115144-35-9
Molecular Formula: C11H7N2O3S2.K
Molecular Weight: 318.42
Purity >99% (chemical and optical purity)
How to make D-luciferin stock solution and work solution:
To prepare solution for in vitro bioluminescent assay: Make a 200x D-luciferin stock solution (30 mg/ml) in sterile deionized water. Mix gently by inversion until the chemical is completely dissolved. Make a D-Luciferin working solution (150 ug/ml final concentration) in pre-warmed culture medium.
To prepare solution for in vivo imaging: Make a D-luciferin stock solution (15 mg/ml) in DPBS. Mix gently by inversion until the compound is completely dissolved. Filter sterilize the solution through a 0.2 um filter. Inject animals at 10 ul/g of body weight or 1.5 mg D-luciferin for a 10 g mouse.
pH stability of D-luciferin potassium salt: If pH>6.3, it may be converted to L-luciferin through a slow racemization.
Storage of D-luciferin potassium salt in powder and solution: It may form dehydroluciferin in the presence of ultraviolet light and/or moisture. The lyophilized powder should be stored in the dark at -20°C. For in vivo and in vitro use, the stock solution after reconstitution with PBS or deionized water can be stored at 4°C with protection from light for 3 weeks, and at -20°C for 3 months. Please contact us if you need protocol, datasheet, or SDS of D-luciferin potassium salt. Quality of our D-luciferin potassium salt has been proven by a number of publications citing our product.
References:
1. “D-luciferin potassium salt (catalogue #MB102, Syd Labs Inc., Natick, MA) was resuspended in phosphate-buffered saline (PBS) to a final concentration of 20 mg/ml. Mice were dosed with 200 μL luciferin substrate intraperitoneally and waited for 10 minutes to allow for distribution of substrate prior to image acquisition.” Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer. David Cunningham, Qiuyang Zhang, Sen Liu, Keshab R Parajuli, Qiang Nie, Lin Ma, Allen Zhang, Zhenbang Chen, Zongbing You. Am J Clin Exp Urol. 2018; 6(3): 114–122.
2. “To monitor the condition of brain metastasis in the mice, bioluminescent imaging (Caliper Life Sciences, Waltham, MA, USA) was used. For this, mice were injected with D-luciferin potassium salt (SYD Labs, Inc., Natick, MA, USA) at a dose of 150 mg/kg i.p., anesthetized, and then placed into the Xenogen IVIS® spectrum imaging system (PerkinElmer, Boston, MA, USA) at 10 min after D-luciferin potassium salt injection.” Cucurbitacin E inhibits the Yes?associated protein signaling pathway and suppresses brain metastasis of human non?small cell lung cancer in a murine model. Hsu PC, Tian B, Yang YL, Wang YC, Liu S, Urisman A, Yang CT, Xu Z, Jablons DM, You L. Oncol Rep. 2019 Aug;42(2):697-707.
3. “BLI was performed using a hybrid optical/X-ray scanner (IVIS Lumina XRMS In Vivo Imaging System, PerkinElmer). Mice were anesthetized with isofluorane (2% in 100% oxygen) and received a 150 μL intraperitoneal injection of D-luciferin (30 mg/mL; Syd Labs, Inc., MA, USA), and BLI images were captured for up to 35 minutes after injection.” Cellular MRI Reveals Altered Brain Arrest of Genetically Engineered Metastatic Breast Cancer Cells. Parkins KM, Hamilton AM, Dubois VP, Wong SM, Foster PJ, Ronald JA. Contrast Media Mol Imaging. 2019 Jan 8;2019:6501231.
Questions and Answers:
Question: Do you provide free sample of your D-luciferin potassium salt?
Answer: Sorry that we do not provide free sample. You can place an order and we will hold the invoice until you tell us that you like our product. You do not need to pay us if you do not like the product. It is an alternative to free sample.
Question: Can your D-luciferin potassium salt be injected to animals such as mice for in vivo study?
Answer: Yes if you filter sterilize the D-luciferin solution before animal injection. Most of our clients use D-luciferin potassium salt for in vivo imaging, as described in References above.
Question: Is D-luciferin potassium salt water soluble?
Answer: Yes, D-luciferin potassium salt is water soluble though its water solubility is inferior to D-luciferin sodium salt.
Question: How is pH stability of D-luciferin potassium salt?
Answer: Its optimal pH is 6.0 to 6.3. If pH>6.3, it may be converted to L-luciferin through a slow racemization. L-luciferin competitively inhibits the luciferase-catalyzed chemical reaction of D-luciferin producing luminescence. Because most clients use water or DPBS to dissolve D-luciferin without pH adjustment, the solution should be stored in -20C and used in no longer than 3 months.
Question: Do you ship your D-luciferin potassium salt to Europe by DHL or FedEx?
Answer: Yes, normally FedEx and sometimes DHL if clients request.
Question: For $1599, can you ship us 10 vials with 1 g/vial?
Answer: Yes.
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Additional information
Ship from Country | USA |
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Size | 1 g, 10 g |