Ponceau S Protein Staining Solution
$50.00
BIO-HELIX Ponceau S Protein Staining Solution (catalog No. PS003-B500ML), Ponceaus S Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.
- Product Details
- Description
- Additional information
- More Offers
| Catalog No. | PS003-B500ML |
|---|---|
| Product Name | Ponceau S Protein Staining Solution |
| Supplier Name | BIO-HELIX |
| Brand Name | BIO-HELIX |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
PS003-B500ML: Ponceau S Protein Staining Solution
Ponceaus S Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.
Figure 1 Ponceau S solution can be used to evaluate for total protein amount or transfer efficiency on nitrocellulose and PVDF membrane. The PVDF membrane stained Ponceau S for 5 mins and washed with ddH2O for 3 mins. It provides visible pink bands.
Lane 1, 13: Prestained Protein Ladder #PM019-0500
Lane 2-7: 2X Dilutions of Unstained Protein Ladder #PMU12-0500
Lane 8-12: 2000, 1000, 500, 100, 50 ng of BSA
Required materials but not provided
Container: box for gel staining
Shaker: orbital or rocking shaker
Reaction Setup
1、After the SDS-PAGE Gel has been transferred to a nitrocellulose or PVDF membrane, place the transferred membrane in a staining container.
2、Submerge the membrane in a proper amount of Ponceau S Protein Staining Solution that is enough to cover the whole membrane. Lightly agitate the staining container for 5 to 15 minutes at room temperature. Protein bands will be stained and start becoming visible in 5 to 15 minutes.
3、Wash away the excessive stain on the membrane with DI water until the protein bands are clear.
4、For the further membrane blocking step, remove the Ponceau S Staining Solution from the membrane by placing the membrane in 20 ml 0.1% NaOH for 1 to 2 minutes then rinse in DI water for 1 to 2 minutes.
Important notes
The Ponceau S Protein Staining Solution is provided as a ready-to-use solution, there is no need to dilute before use.
Please remember our product information: Ponceau S Protein Staining Solution catalog number: PS003-B500ML BIO-HELIX
Additional information
| Ship from Country | USA |
|---|---|
| Size | 500 ml |
