In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg

Catalog No.	PA007131.05
Product Name	In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	hIgG4 Isotype Control, Human IgG4 Isotype Control, hIgG4 S228P, hIgG4 SPLEPG Mutant
Summary	The In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg was produced in mammalian cells.
Clone	4F17.
Isotype	human IgG4, kappa.
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution (1 mg/ml) of 1x PBS.
Endotoxin	< 0.05 EU per 1 mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg and mutants are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant human IgG4 kappa isotype control mutants, hIgG4 S228P and SPLEPG, good for in vivo and in vitro studies. Low or no specific binding to human samples tested. Humanized variable regions and hIgG4 kappa constant regions. The chimeric versions and premium grade (endotoxin ≤0.05 EU/mg) are available.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card.

Description

PA007131.05: In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg

Background

Isotype control antibodies (negative control antibodies or reference antibodies) are frequently used in various antibody-related in vivo and in vitro experiments such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Isotype control antibodies are used as negative controls of primary antibodies to check presence of non-specific binding to Fc receptors on the cell surface, other cellular proteins, carbohydrates, and lipids. Thus, the isotype control antibody normally matches the host species and isotype, including light chains (such as human IgG1 kappa antibody), concentration, and conjugation format of the experimental primary antibody.

The following mutations have been introduced to alter the constant regions of an IgG1 antibody to change its immunologic properties by Fc interaction with host proteins:

hIgG1 heavy chain R214K
hIgG1 heavy chain R214K/N297A
hIgG1 heavy chain L234A/L235A
hIgG1 heavy chain L234F/L235E/P331S
hIgG1 heavy chain L235A/G237A
hIgG1 heavy chain D356E/L358M
hIgG1 heavy chain F126L
hIgG1 heavy chain D265A
hIgG1 heavy chain F243L/R292P/Y300L/V305I/P396L
hIgG1 heavy chain S239D/I332E
hIgG1 heavy chain S239D/I332E/A330L
hIgG1 heavy chain S298A/E333A/K334A
hIgG1 heavy chain G236A/S239D/I332E
hIgG1 heavy chain K326W/E333S
hIgG1 heavy chain S267E/H268F/S324T
hIgG1 heavy chain E345R/E430G/S440Y
hIgG1 heavy chain N297A
hIgG1 heavy chain N297Q
hIgG1 heavy chain N297G
hIgG1 heavy chain L235E
hIgG1 heavy chain L234A/L235A
hIgG1 heavy chain M252Y/S254T/T256E
hIgG1 heavy chain M428L/N434S
hIgG1 heavy chain S267E/L328F
hIgG1 heavy chain N325S/L328F

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody

In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

Please remember our product information: In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg: PA007131.05 Syd Labs

Additional information

Ship from Country	USA
Size	25 mg, 5 mg

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