HiScript II Q RT SuperMix for qPCR R222-01
$290.00
Vazyme HiScript II Q RT SuperMix for qPCR (R222-01) is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency.
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| Catalog No. | R222-01 |
|---|---|
| Product Name | HiScript II Q RT SuperMix for qPCR R222-01 |
| Supplier Name | Vazyme International LLC |
| Brand Name | Vazyme |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card. |
Description
R222-01:HiScript II Q RT SuperMix for qPCR
Product Introduction
HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for two-step qRT-PCR. The 5 × HiScript qRT SuperMix contains all components required for reverse transcription. With the addition of template RNA and ddH2O, the reaction can proceed quickly. The volume of template RNA can be up to 80% of the total volume, which is very suitable for reverse transcription reaction at low concentration of template RNA. 5 × HiScript II qRT SuperMix will not freeze at -20℃.
This product has been specially optimized for qPCR. Oligo (dT)23VN has stronger anchoring ability for poly A+ mRNA than Oligo (dT)18, resulting in higher reverse transcription efficiency. With the optimized proportion of Random primers/Oligo (dT)23VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results. The RT product is compatible with dye-based and probe-based qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.
Product Advantages
Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher;
Anchored Oligo (dT)23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis;
The ready-to-use SuperMix is easy to use and time-saving
Storage
Store at -30 ~ -15℃ for up to 12 months, and transport at ≤0℃.
Please remember our product information: HiScript II Q RT SuperMix for qPCR catalog number: R222-01 Vazyme.
Additional information
| Ship from Country | USA |
|---|---|
| Size | 100 rxns (20 µl/rxn) |