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VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2
VAHTS Stranded mRNA-seq Library Prep Kit for Illumina® V2 is specially designed for the preparation of stranded transcriptome libraries for next generation sequencing (NGS) platforms of Illumina®.
VAHTS Stranded mRNA-seq Library Prep Kit for Illumina® V2 is specially designed for the preparation of stranded transcriptome libraries for next generation sequencing (NGS) platforms of Illumina®. This kit is suitable for the library preparation starting from 50 ng-4 µg of intact total RNA of
eukaryotes (e.g. animal, plant, or fungal). Different from non-stranded transcriptome library prep kits, this kit enables the insertion of dUTP during the
2nd strand synthesis of cDNA. The double-strand cDNA is digested with uracil-DNA glycosylase (UDG) to remove the second strand containing dUTP
before library enrichment. As a result, only information from the 1st strand cDNA is retained. In addition to standard transcriptome information,
strand-specific (e.g. from sense or anti-sense DNA) information can also be obtained from NGS data analysis.
This kit is upgraded from VAHTS Stranded mRNA-seq Library Prep Kit for Illumina®. It significantly simplifies the operation process and improves
library preparation efficiency. It is compatible with lower-input RNA. Libraries of specific sizes, which can be customized, can be obtained after size
selections with magnetic beads.