Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Or leave a message with a formal purchase order (PO) Or credit card.
Size Exclusion HPLC Services
A setup fee may apply depending on number of samples and sample preparation time. The above quoted prices are based on analysis using our standardized methods, proven to work for the majority of antibodies and proteins. Method development options may be necessary for atypical proteins with unique properties.
Protein aggregation may be encountered during various steps of the manufacturing process of biopharmaceuticals, and can potentially cause adverse effects such as an immune response, or impact the drug’s efficacy. Size exclusion HPLC (SE-HPLC) has become an essential analytical tool for the detection and analysis of soluble protein aggregates. The separation mechanism is based on the protein shape and size. Proteins of different sizes elute at different rates through the column, with larger proteins eluting first.
Service of protein aggregation analysis by SE-HPLC includes:
A. Perform size-based separation of proteins/antibodies on an SEC column with UV detection.
B. Detect and measure soluble aggregates and clips/degradation products by area per cent (if present).
- Soluble aggregates and clips in terms of %.
- Presence of conformers.
Sample treatment: Direct injection with UV detection.