Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Or leave a message with a formal purchase order (PO) Or credit card.
SARS-CoV-2 IgG ELISA Kit (S Protein)
Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
EK096: SARS-CoV-2 IgG ELISA Kit (S Protein, Spike Protein)
Application: The human IgG antibody detection kit with indirect ELISA method is used for qualitative detection or semi-quantitative measurement of human IgG antibodies specific for the SARS-CoV-2 spike protein, S1 subunit in serum or plasma.
Predicted molecular mass: 160.7 kDa.
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: The recombinant SARS-CoV-2 Spike S protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.
Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
The SARS-CoV-2 spike (S) glycoprotein plays the most important roles in viral attachment, fusion and entry. The virus causing Coronavirus Disease 2019 (COVID-19) was named 2019 novel coronavirus (2019-nCoV) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). There are four major structural proteins in SARS-CoV-2, spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins.
There are two subunits, S1 and S2, in the SARS-CoV-2 spike (S) glycoprotein. The furin cleavage site at the boundary between the S1/S2 subunits is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs. The S1 subunit, the N-terminal 14-685 amino acids of S protein, contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM). The C-terminal S2 subunit contains fusion peptide (FP), heptad repeat 1 (HR1), heptad repeat 2 (HR2), transmembrane domain (TM), and cytoplasmic domain (CP). The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit.