2 × Rapid Taq Master Mix

2 × Rapid Taq Master Mix

RoomTemp Sample Lysis Kit

$140.00
In stock
CatalogP073

The RoomTemp Sample Lysis Kit is a simple and fast blood lysis kit used at room temperature. The kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that rapidly destroy the proteins and structures of cell membrane, and can fully release genomic DNA in cells. Stabilizing Buffer contains protective proteins and stabilizing factors to eliminate the effect of the inhibitors in the lysed sample
on the downstream reaction such as qPCR and PCR, allowing long-term storage of the lysed DNA solution. Applicable blood sample types include fresh blood, cryopreserved blood, and conventional anticoagulant blood (EDTA, citrate, sodium heparin, etc.). Genomic DNA can be released from whole blood samples by lysis for 3 min at room temperature. And the lysed DNA solution can be directly used as a template for downstream applications such as SNP detection by probe-based Taqman, quantifcation by probe-based qPCR, PCR amplifcation. No need for complicated extraction operations, it can achieve the same effect as the traditional genome extraction method. In addition to blood, this kit is also compatible with a variety of samples such as lysed FTA cards, buccal swabs, and plant tissues.

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