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Retrovirus Production Services
No DNA using our proprietary retrovirus plasmids is delivered.
Addtional fee for DNA scale up is charged for BS084B-D.
Retrovirus purification: ultracentrifugation. (optional) Filtration, chromatography.
Quality control: titering by qPCR. (optional) Endotoxin measurement and reduction, HT1080 assay.
Our custom retrovirus production services include: 1) construction of a retrovirus plasmid vector; 2) retrovirus packaging; 3) retrovirus purification; 4) retrovirus titration. Our retrovirus production team has constructed, packaged, and purified hundreds of retroviruses from small crude scale to large purified scale for worldwide clients in more than a decade. We have always optimized our retrovirus production systems, including retrovirus plasmid construction and low endotoxin plasmid preparation, host cells for retrovirus packaging, cell culture medium and retrovirus packaging process, small and large scale retrovirus purification process.
Retroviruses are popular gene therapy vectors because they stably integrate the DNA copy of their genome into the host chromosome during their replication cycle. Advances in design of retroviral vectors have resulted in greater degree of biosafety, expanded host range, and increased stability of the virus particles. Retroviral vectors have been widely used in the ex vivo gene therapy protocols to correct the liver diseases in a wide variety of species. Syd Labs provides services for the construction and production of vectors based on murine retrovirus.
Molecular construction of a retrovirus plasmid vector and mini-scale retrovirus production.
Gene-of-interest (GOI) sequences are subcloned into our proprietary lentivirus production plasmids or plasmid vectors that clients provide, and transduced into mammalian cells.
A. Retrovirus stock (5 ml, 1 x 10^5-6 TU/ml or 1 x 10^8-9 LP/ml);
B. Study report.
Syd Labs also provides the following virus production (virus vector construction, virus packaging, virus amplification, virus purification, and virus titering) services:
Lentivirus Production Services
Adeno-associated Virus (AAV) Production Services
Adenovirus Production Services
Adenovirus Production Services for Toxic Genes
Adenovirus Production Services for Fiber-modified Ad5-RGD and Ad5/35