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Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein
Conditions of optimal recombinant SARS-CoV-2 Spike S1-Fc protein performance should be determined experimentally by the investigator.
BP003056: Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein
Source: Recombinant SARS-CoV-2 Spike S1 protein (Gln14-Arg683) was expressed in mammalian cells with a Fc tag and Avi at the C-terminus.
Predicted molecular mass: 102.6 kDa. Due to glycosylation, the recombinant SARS-CoV-2 Spike S1 protein migrates to 130-140 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Activity: Immobilized recombinant human ACE2, His Tag at 0.5 ug/ml (100 ul/well). Dose response curve for the recombinant SARS-CoV-2 Spike S1-Fc-Avi protein with the EC50 of 0.1 ug/ml determined by ELISA.
Formulation: The recombinant SARS-CoV-2 Spike S1-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.
Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
There are two subunits, S1 and S2, in the SARS-CoV-2 spike (S) glycoprotein which plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of virus entry inhibitors, neutralizing antibodies, and vaccines. The S1 subunit, the N-terminal 14–685 amino acids of S protein, contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM). The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit.
Both His-tagged and Fc-fused recombinant SARS-CoV-2 S1 proteins are available with an Avi site, which is for biotinylation. The biotinylated recombinant S1 proteins are also avaible. Please ask for a quote if you need protein mutants or a protein with different modification or components.
To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services