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Recombinant Antibody (scFv) Production Services
No DNAs using our recombinant antibody expression vectors will be delivered.
All yields are estimates and not guaranteed.
Prices of BS079D to BS079I do not include DNA scale-up. Fees are based on transient production using HEK 293 cells and protein A purification. Fees are higher if CHO cells or other purification methods are used.
Our recombinant antibody (single-chain variable fragment, scFv-Fc) production services include: 1) PCR amplification, or optimization and synthesis of antibody variable regions (VH and VL) or scFv-Fc antibodies for molecular construction of recombinant scFv-Fc antibodies with our proprietary antibody expression vectors; 2) transient production of recombinant antibodies in HEK 293 cells; 3) purification of recombinant antibodies. Made as a single protein, scFv-Fc retains the specificity of the original immunoglobulin, and in certain applications is preferable to whole IgG in construction speed, production yield, and engineering flexibility.
1. Synthesizing antibody variable regions (both VH and VL) or sequences that clients provide:
Including sequence optimization, for free, to meet your goals such as recombinant antibody expression at high and reliable levels.
2. Construction, expression, and purification of scFv-Fc (choice of human or mouse IgG1-Fc backbone; other recombinant IgG-Fc construction is available upon request):
* Connect VH with VL using a short linker peptide of 10-25 amino acids;
* Construct the recombinant scFv-Fc;
* Transiently express the recombinant scFv-Fc protein in HEK 293 cells;
* Purify the recombinant scFv-Fc protein with protein A. Please request if multiple purification methods are required.
The Fc options include but are not limited to:
* Human IgG1-Fc, IgG2-Fc, IgG3-Fc, IgG4-Fc;
* Mouse IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc;
* Rat IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc;
* Rabbit IgG-Fc;
* Canine IgG-Fc.
If you provide us the expression vector with your gene already cloned into, we can perform small-scale pilot expression and protein purification by transient transfection into 293 cells to evaluate expression level and yield of the protein of your interest.
Fast turnaround (BS079A, B, and C):
* 1-2 weeks for codon optimization and synthesizing antibody variable regions.
* 1-2 weeks for molecular construction of recombinant scFv-Fc antibodies using E.coli.
* 1-2 weeks for expressing and purifying recombinant scFv-Fc antibodies using mammalian expression systems, CHO or HEK 293 cells.
* BS079D: 0.03 liter transient production, estimated yield 0.5 - 12 mg, average 1.5 mg, 2 weeks;
* BS079E: 0.1 liter transient production, estimated yield 3 - 110 mg, average 14 mg, 2 weeks;
* BS079F: 0.5 liter transient production, estimated yield 12 - 300 mg, average 75 mg, 2 weeks;
* BS079G: 2 liter transient production, estimated yield 50 - 2000 mg, average 250 mg, 3 weeks;
* BS079H: 10 liter transient production, estimated yield 120 - 5000 mg, average 1100 mg, 3 weeks.
A. Purified scFv-Fc protein (from HEK 293 transient production);
B. Study report.
We also provide the following antibody sequencing and production services:
Clone and sequence hybrimona antibody VH and VL
Construction, expression, and purification of whole IgG antibodies
Generation of stable cell lines expressing antibodies
scFv phage library construction service
ScFv Phage Library Screening Service