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Recombinant Antibody (IgG) Production Services
No DNAs using our recombinant antibody expression vectors will be delivered.
All yields are estimates and not guaranteed.
Prices of BS046D to BS046H do not include DNA scale-up. Fees are based on transient production using HEK 293 cells and protein A purification. Fees are higher if CHO cells or other purification methods are used.
Our recombinant antibody production services include: 1) PCR amplification, or optimization and synthesis of antibody variable regions (VH and VL) or whole IgG antibodies for molecular construction of recombinant IgG antibodies with our proprietary antibody expression vectors; 2) transient production of recombinant antibodies in mammalian expression systems, such as CHO and HEK 293 cells; 3) purification of recombinant antibodies. Different antibody constant regions can be expressed with the same antibody variable region to produce chimeric antibodies, which is called isotype switching or class switching. For example, the IgM isotype can be switched to the IgG isotype. The switched chimeric antibody keeps the antigen affinity resulting from the same variable region but interacts with different effector molecules because of the different constant regions.
1. Synthesizing antibody variable regions (VH and VL) or whole IgG antibodies:
Including sequence optimization, for free, to meet goals such as recombinant antibody expression at high and reliable levels.
2. Construction, expression, and purification of whole IgG antibodies (choice of human or mouse IgG1 backbone; other recombinant IgG construction is available upon request):
* Fuse VH and VL into human or mouse IgG constant region backbone in antibody expression vectors;
* Transiently express the recombinant IgG antibody in HEK 293 cells;
* Purify the recombinant IgG antibody with Protein A. Please request if other purification method or multiple purification methods are required.
The IgG backbone options include but are not limited to:
* Human IgG1, IgG2, IgG3, IgG4;
* Mouse IgG1, IgG2a, IgG2b, IgG3;
* Rat IgG1, IgG2a, IgG2b, IgG3;
* Rabbit IgG;
* Canine IgG.
If you provide us the expression vector with your gene already cloned into, we can perform small-scale (30 ml) pilot expression and protein purification by transfection into HEK 293 cells to evaluate transient expression level and yield of the protein of your interest.
Fast turnaround (BS046A, B, and C):
* 1-2 weeks for codon optimization and synthesizing antibody variable regions.
* 1-2 weeks for molecular construction of recombinant scFv-Fc or IgG antibodies using E.coli.
* 1-2 weeks for expressing and purifying recombinant scFv-Fc or IgG antibodies using HEK 293 cells.
* BS046D: 0.03 liter transient production, estimated yield 0.5 - 10 mg, average 2 mg, 2 weeks;
* BS046E: 0.1 liter transient production, estimated yield 2 - 50 mg, average 10 mg, 2 weeks;
* BS046F: 0.5 liter transient production, estimated yield 10 - 250 mg, average 50 mg, 2 weeks;
* BS046G: 2 liter transient production, estimated yield 40 - 2000 mg, average 200 mg, 3 weeks;
* BS046H: 10 liter transient production, estimated yield 200 - 5000 mg, average 1000 mg, 3 weeks.
A. Purified antibody (from HEK 293 transient production);
B. Study report.
Clone and sequence hybrimona antibody VH and VL
Construction, expression, and purification of single-chain antibodies (scFv-Fc) antibodies
Generation of stable cell lines expressing antibodies
Recombinant IgG Reference Antibodies:
Recombinant Human IgG1 Reference Antibody
Recombinant Human IgG2 Reference Antibody
Recombinant Human IgG4-S228P Reference Antibody
Recombinant Mouse IgG1 Reference Antibody
Recombinant Mouse IgG2a Reference Antibody
Recombinant Mouse IgG2b Reference Antibody