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Recombinant 2019 nCOV Spike S1
2019-nCoV Spike S1 is expressed from mammalian with a His tag and Avi at the C-terminal.
It contains Gln 14-Arg 683.
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120KDa based on Bis-Tris PAGE result.
Less than 1EU per ug by the LAL method.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). Normally 5 % trehalose is added
as protectant before lyophilization.
The product should be stored at -70℃ or -20℃.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin- converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.