Recombinant 2019 nCOV Spike RBD(Fc-Avi)

Source
2019-nCoV S protein RBD is expressed from mammalian with a Fc tag and Avi at the C-terminal.
It contains Arg319-Asn532.

Accession
QHD43416.1

Molecular Weight
The protein has a predicted MW of 51.7kDa. Due to glycosylation, the protein migrates to 60-62KDa based on Bis-Tris PAGE result.

Endotoxin
Less than 1EU per ug by the LAL method.

Purity
> 95% as determined by Bis-Tris PAGE   
> 95% as determined by HPLC

Formulation
Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). Normally 5 % trehalose is added
as protectant before lyophilization.

Storage
The product should be stored at -70℃ or -20℃. .

Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin- converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.