Conditions of optimal RANKL protein performance should be determined experimentally by the investigator.
Receptor activator of nuclear factor kappa-B ligand (RANKL or RANK Ligand) , also referred to as TNFSF11, TRANCE, OPGL and CD254, is a member of the tumour necrosis factor (TNF) family of cytokines that binds to its receptor RANK to control osteoclast differentiation, activation and survival (1-3). RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.
1. Kong, Y. Y. et al. OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis. Nature 397, 315-323 (1999).
2. Lacey, D. L. et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 93, 165-176 (1998).
3. Anderson, D. M. et al. A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function. Nature 390, 175-179 (1997).
BP000050-GD13: Recombinant Human RANKL Protein
Source: E. coli-derived.
The activity of the RANKL protein (RANK ligand, TNFSF11, TRANCE, OPGL, CD254) is calculated by its ability to induce osteoclast differentiation on RAW 264.7 mouse macrophages. The ED50 for this effect is 2-12 ng/ml.
Endotoxin Level: <1.0 EU per ug of the RANKL protein by the LAL method.
Purity > 95%, by SDS-PAGE and SEC-HPLC.
Formulation: In PBS with 0.1% BSA.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
RANK polyclonal antibody