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N-terminal Protein Sequencing Service
The prices described above are based on analysis using our standardized methods, proven to work for the majority of antibodies and proteins. Method development options may be necessary for atypical proteins with unique properties.
A setup fee may apply depending on the order volume.
N-terminal Edman sequencing of proteins or peptides can be used to determine the N-terminal amino acid residues of purified protein samples in addition to cleavage sites, and to identify an unknown protein or confirm the identity of a known protein, or to design oligonucleotide probes. Advantages of N-terminal Edman sequencing include high sensitivity, even with picomoles of protein. Proteins can be sequenced in solution, gels or on PVDF blots.
General procedure of N-terminal protein sequencing:
N-terminal Edman sequencing of proteins will be performed with five to twenty residues (degradation steps), depending on information needed as follows:
A. 5 residues are recommended for determination of a cleavage site in a known protein.
B. 5-10 residues are recommended to confirm the identity of an isolated known protein.
C. 10-20 residues are recommended to design oligonucleotide probes.
D. 15 or more residues are recommended to identify an unknown protein.
Sample size: 50 ug protein;
Formulation: freeze-dried or dissolved in 1X PBS or equivalent buffers;
Purity: > 95%.
A. Report with N-terminal amino acid sequences of purified protein samples.
Note: if N-terminal of heavy chain is blocked, N-terminal sequence may not be obtained.