Immunohistochemistry Services

Immunohistochemistry Services

Antigen-antibody Affinity Measurement Service

Antigen-antibody Affinity Measurement Service

Monoclonal Antibody Immunoconjugation Service

CatalogBS039
Grouped product items
Product Name Quantity
Pilot Study Phase I: Generation of antibody immunoconjugates (Catalog No.: BS039A)
$3,500.00
Pilot Study Phase II: In vitro Bioassay (Catalog No.: BS039B)
$9,000.00
Medium-size Production Phase I: Generation of antibody immunoconjugates (Catalog No.: BS039C)
$3,500.00
Medium-size Production Phase II: In vitro Bioassay (Catalog No.: BS039D)
$8,000.00
Medium-size Production Phase III: In vivo animal study (Catalog No.: BS039E)
$22,500.00

1. Pilot Study

Material required
3~5 mg purified monoclonal antibody needed

Phase I Generation of antibody immunoconjugates
? Exchange the antibody buffer to the conjugation buffer
? Activation of the antibody
? Conjugation of the antibody to TWO different drugs at TWO different molar ratio
? Purification of the immunoconjugates
? Filter-sterilization

Phase II In vitro Bioassay
? Seeding the cells in 96-well plate, up to THREE different cell lines
? Adding the immunoconjugates at various concentrations.
? Performing MTT assay on day 5.
? Repeat the bioassay twice

2. Medium-size Production

Material required
5~10 mg purified monoclonal antibody needed

Phase I Generation of antibody immunoconjugates
? Exchange the antibody buffer to the conjugation buffer
? Activation of the antibody
? Conjugation of the antibody to ONE drug at TWO different molar ratio
? Purification of the immunoconjugates
? Filter-sterilization

Phase II In vitro Bioassay
? Seeding the cells in 96-well plate, up to THREE different cell lines
? Adding the immunoconjugates at various conc.
? Performing MTT assay on day 5.
? Repeat the bioassay once
? Sending out 2 mg immunoconjugate.

Phase III In vivo animal study
? Innoculate 30 nude mice with the right number of tumor cells
? Once the tumors reach 10 mm, 5 mice per group will be injected with various amount of immunoconjugate, naked antibody, unconjugated drug and buffer
? Tumor size will be measured every 3 or 4 days
? At the end of the test, the tumors will be removed and weighed individually.
? Repeat the animal study twice.

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