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Lentivirus Production Service
No DNA using our proprietary lentivirus plasmids is delivered.
Addtional fee for DNA scale up is charged for BS084B-D.
Lentivirus purification: ultracentrifugation. (optional) Filtration, chromatography.
Quality control: titering by p24 ELISA. (optional) Endotoxin measurement and reduction, qPCR, HT1080 assay.
Our complete custom lentivirus production services include: 1) lentivirus plasmid vector construction; 2) lentivirus packaging; 3) lentivirus purification; 4) lentivirus titering. Our lentivirus production team has constructed, packaged, and purified thousands of lentiviruses from small crude scale to large purified scale for worldwide clients in more than a decade. We have always optimized our lentivirus production systems, including lentivirus plasmid construction and low endotoxin plasmid preparation, host cells for lentivirus packaging, cell culture medium and lentivirus packaging process, small and large scale lentivirus purification process.
Recombinant lentiviral vectors (rLV) are widely used in gene transfer and genome editing for expression or silencing of a gene (lentiviral based stable cell line generation, knock-out and knock-in cell line generation with CRISPR genome editing, and Chimeric Antigen Receptor T (CAR T)-cell engineering with AAV or alone) due to the following advantages:
- lentiviral vectors can accommodate long sequences;
- lentiviruses seem to be non-immunogenic due to lack of viral coding sequence transfer;
- lentiviruses are able to transduce non-dividing cells;
- proteins can be stably expressed due to integration into the cell chromosome.
BS084A. Molecular construction of a lentivirus vector and mini-scale lentivirus production.
Gene-of-interest (GOI) sequences are subcloned into our proprietary lentivirus production plasmids or plasmid vectors that clients provide, and transduced into mammalian cells.
Lentivirus stock (5 ml, 1 x 10^5-6 TU/ml or 1 x 10^8-9 LP/ml).
Lentiviral titers are determined by p24 ELISA (LP/ml), and/or fluorescent positive cells (TU/ml).
References of lentivirus production protocols, lentivirus purification protocols, and lentivirus titration protocols:
Lentivirus production protocol
A polyethyenimine (PEI) transfection protocol used to produce lentivirus from a lentiviral vector transfected into Lenti-X 293T cells.
Lentivirus production and transfection protocol
A prototol for shRNA lentivirus production using HEK293T cells and FuGENE.
Protocol for generation of lentivirus
A standard lentivirus generation protocol.
The procedure of lentivirus production sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provide comprehensive lentivirus production services if the project is difficult for you.
We index "References of lentivirus production protocols, lentivirus purification protocols, and lentivirus titration protocols" for your information only. It does not necessarily mean that we agree with all of them. You rather than Syd Labs takes full responsibility for using any information described here.)
Syd Labs provides the following lentivirus-related services:
Virus Titer Test Services for Lentiviruses and Retroviruses
Lentiviral Based Stable Cell Line Generation Services
Knock-out and Knock-in Cell Line Generation with CRISPR Genome Editing
Chimeric Antigen Receptor T-Cell Engineering
Syd Labs also provides the following virus production services (virus vector construction, virus packaging, virus amplification, virus purification, and virus titering):
Retrovirus Production Services
Adeno-associated Virus (AAV) Production Services
Adenovirus Production Services
Adenovirus Production Services for Toxic Genes
Adenovirus Production Services for Fiber-modified Ad5-RGD and Ad5/35