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Intact Mass Analysis by LC-MS Services
A setup fee may apply depending on the volume of clents' order.
Mass spectrometry (Bruker Q-TOF) is used to determine an accurate molecular weight of a protein/antibody of interest. The protein/antibody is denatured and then subjected to liquid chromatography-mass spectrometry (LC-MS) analysis. Antibody samples are reduced for separate analysis of light chains and heavy chains with the spectrum data de-convoluted for final intact mass spectra. Depending on the nature of samples and/or upon client’s request, samples can also be analyzed without reduction and denaturation in order to acquire the global profile of antibodies.
Besides reporting the accurate molecular weight, intact mass analysis can be used to define approximate glycoprofiling of major N-linked oligosaccharides such as G0f, G1f and G2f. For example, upon request, the intact mass of de-glycosylated samples can be analyzed following removal of N-linked glycans using an enzyme such as PNGase-F. Also upon request, the approximate assessment of truncations and major post-translational modifications (PTMs) such as oxidation, N-terminal pyroglutamate formation, and C-terminal Lys processing may be determined depending on the complexity of the modifications and the nature of samples.
- Accurate MW determination of LC & glycosylated HC (PNGaseF treatment prior to LC-MS is also possible).
- Quick glycoprofiling of major N-linked oligosaccharides (G0f, G1f and G2f).
- Quick identification of truncations and major PTMs such as oxidation, N-term pyroglutamate formation and C-term Lys processing.