In Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody

In Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody

$295.00$2,950.00

In stock

$295.00$2,950.00

Recombinant Syrian hamster IgG1 isotype control antibody good for in vitro and in vivo studies. Low or no specific binding to mouse samples tested. Mouse variable regions and Syrian hamster IgG1 kappa constant regions. Silent Fc hamster IgG isotype controls available.

SKU: PA007149
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Catalog No. PA007149
Product NameIn Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody
Supplier Name Syd Labs, Inc.
Brand Name Syd Labs
Clone 12B9.
Source/Host The recombinant Syrian hamster IgG1 isotype control antibody (Syrian hamster IgG isotype control) was produced in mammalian cells.
Specificity/Sensitivity The in vivo grade recombinant Syrian hamster IgG1 isotype control antibody does not bind to any known target.
Applications an isotype-matched negative control for Syrian hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form Of Antibody 0.2 μM filtered solution of PBS, pH 7.4.
Endotoxin Less than 1 EU/mg of protein as determined by LAL method.
Purity >95% by SDS-PAGE under reducing conditions.
Shipping The in vivo grade recombinant Syrian hamster IgG1 isotype control antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Order Offline Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007148: In Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody

Recombinant IgG1 Monoclonal Antibody.

Background

Hamster IgG antibodies are classified to three subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which kappa is the most abundant in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

Syrian hamster IgG1 antibodies can be purified by the protein G beads, and relatively lower yield by the protein A beads.

Both Syrian hamster IgG1 isotype control and Armenian hamster IgG1 isotype control (natural and engineered hamster IgG1 isotype controls) are useful for in vivo functional study using mouse and rat research model among a number of in vitro and in vivo research applications from ELISA, Western Blot (WB) to cell-based assays. The amino acid and DNA sequences of Syrian hamster IgG antibodies are much more different from mouse sequences than rat ones. Most of Syrian hamster antibodies are used to study mouse and rat target proteins. The ideal recombinant Syrian hamster IgG1 isotype control for in vivo use has low or no specific binding to any mouse and rat sample, and murinized or completely mouse variable regions in addition to the Syrian hamster IgG1 kappa constant regions.

There are more and more IgG antibody formats with Fc engineered. The formats with the Fc silenced, Fc silent, or Fcs with silent effector function is the most popular. Thus, we provide Fc silent effector function Syrian hamster IgG isotype controls, including Syrian hamster IgG1 and IgG2 kappa isotype controls.

Please contact us to ask for a quote for the Fc silenced Syrian hamster IgG1 isotype control mutants, Syrian hamster IgG1 isotype control and Fc silent mutants with Avi-, His-, and Flag-tags, and biotinylated Syrian hamster IgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the Syrian hamster IgG isotype control and Fc effector-silent mutants are available.

Questions and Answers:

Question: Why do you use mouse variable regions in your recombinant Syrian hamster IgG isotype controls?
Answer: The isotype control is normally used to differentiate any non-specific background signal from the specific antibody signal, including the immunogenicity if used in animals. Most in vivo functional grade Syrian hamster IgG antibodies are used to study mouse target proteins. Many scientists use Syrian hamster IgG isotype controls with Syrian hamster antibody variable regions assuming that they induce the same immune response in mice, if there is any, as the Syrian hamster antibodies of interest. However, two Syrian hamster variable regions including the one in the Syrian hamster IgG isotype control do not necessarily induce the same immune response in mice because in some cases, even one amino acid difference can make difference. Mouse variable regions can be used to show the potential immunogenicity induced by the Syrian hamster variable regions in the Syrian hamster IgG antibodies to test. Thus, it is better to use the mouse variable region in the Syrian hasmter IgG isotype control. We also have the Syrian hamster IgG isotype controls with the Syrian hamster IgG variable regions. Please contact us if you are interested.

Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant Syrian hamster IgG isotype controls. There is significant advantage for the in vitro method. First, we do not use mouse ascites for antibody production. Hamster hybridoma cell lines usually adapt poorly to in vitro conditions, and generate ascites in immunocompromised mice rather than hamsters. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies. Fourth, we can easily switch the isotype with different antibody constant regions, and generate Fc mutations such as D265A which is used in therapeutic antibody research. It is better to use the same isotype control mutation.

Question: What is the target of your recombinant Syrian hamster IgG1 kappa isotype control antibody?
Answer: Similar to our other hamster IgG isotype control antibodies, the recombinant Syrian hamster IgG1 kappa isotype control antibody (catalog # PA007148) does not bind to any protein or chemical, even to those popular antigens for isotype control antibodies such as KLH, TNP, DNP, GST, Hen Egg Lysozyme (HEL), His-tag, Flag, c-myc, HA, and GFP. PA007148 is an engineered Syrian hamster IgG1 kappa antibody without antigen binding activity. We also produce and sell recombinant Syrian hamster IgG1 kappa isotype controls against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, and GFP. Please contact us and ask for them if you are interested.

Question: Is PA007148 your preferred Syrian hamster IgG1 kappa isotype control. Correct?
Answer: Yes because it was engineered to lose the target binding activity. In addition, its production yield is higher so that we can reduce the production cost and our selling prices.

Syd Labs produces a number of in vivo grade isotype control antibodies. Some customers purchase them for in vitro assays such as flow cytometry or immunohistochemistry (IHC) because the prices are too good if compared to those research reagents of antibody isotype controls. Syd Labs provides the following in vivo grade recombinant hamster IgG isotype control antibodies and mutants:
In vivo Grade Recombinant Armenian Hamster IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Armenian Hamster IgG2 Isotype Control Antibody and Mutants

Syd Labs provides the following in vivo grade recombinant human, mouse, and rat IgG isotype control antibodies and mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody

Frequent questions related to “In Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody”:

How to purify hamster antibodies?

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Additional information

Ship from Country

USA

Size

1 mg, 25 mg, 5 mg

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