In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody

In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody

$195.00

In stock

$195.00

In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody. Recombinant anti-HEL isotype control antibodies and Fc silenced mutants from clone HyHEL-10 good for in vitro and in vivo studies. Low or no specific binding to human, mouse, and rat samples tested. “Humanized” means the variable regions were humanized.

SKU: PA007154.m3
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Catalog No. PA007154.m3
Product NameIn vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody
Supplier Name Syd Labs, Inc.
Brand Name Syd Labs
Synonyms Anti-HEL Isotype Control, Recombinant HyHEL-10 Isotype Control, HyHEL-10 Negative Control Antibody
Summary The In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody was produced in mammalian cells.
Clone HyHEL-10.
Isotype mouse IgG1, kappa.
Applications an isotype-matched negative control for anti-HEL mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Immunogen hen egg-white lysozyme (HEL).
Form Of Antibody 0.2 μM filtered solution of 1x PBS.
Endotoxin Less than 1 EU/mg of protein as determined by LAL method.
Purity >95% by SDS-PAGE under reducing conditions.
Shipping The In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Note Recombinant anti-HEL isotype control antibodies and Fc silenced mutants from clone HyHEL-10 good for in vitro and in vivo studies. Low or no specific binding to human, mouse, and rat samples tested. "Humanized" means the variable regions were humanized.
Order Offline Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card.

Description

PA007154.m3:In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody

Background

The anti-HEL monoclonal antibody (hen egg-white lysozyme) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant mouse IgG1 kappa isotype control (clone number: HyHEL-10) and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.

As an ideal recombinant mouse IgG1 isotype control, the recombinant HyHEL-10 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and has completely mouse variable regions and the mouse IgG1 kappa constant regions. The anti-HEL mouse IgG1 kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered HyHEL-10 isotype control mutants, isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-HEL isotype control and mutants are available.

The in vivo grade recombinant HyHEL-10 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.

In Vivo Grade Recombinant anti-HEL Isotype Control Antibodies (clone HyHEL-10):
In vivo Grade Recombinant HyHEL-10 Mouse IgG1 Isotype Control Antibody Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse Fab Fragment
In vivo Grade Recombinant HyHEL-10 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human Fab Fragment
In vivo Grade Recombinant HyHEL-10 Human IgE Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Human IgM Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat Fab Fragment
In vivo Grade Recombinant HyHEL-10 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Feline IgG1 Isotype Control Antibody

Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody

Please remember our product information: In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody: PA007154.m3 Syd Labs

Additional information

Ship from Country

USA

Size

1 mg

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