In Vivo Grade Recombinant Human IgG1-S267E L328F (SELF) Isotype Control Antibody

Catalog No.	PA007140
Product Name	In Vivo Grade Recombinant Human IgG1-S267E L328F (SELF) Isotype Control Antibody
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	hIgG1 Isotype Control, hIgG1 D265A N297A, IgG1 LALAPG, IgG1 SELF Mutants
Summary	The in vivo grade recombinant human IgG1-S267E L328F (hIgG1 SELF, Fc activated) kappa isotype control mutant (hIgG1 SELF isotype control) was produced in mammalian cells.
Clone	4F17.
Isotype	human IgG1, kappa.
Applications	an isotype-matched negative control for human IgG1 SELF kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In Vivo Grade Recombinant Human IgG1-S267E L328F (hIgG1 SELF) kappa Isotype Control Antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Note	Cheapest in vivo grade recombinant human IgG1 kappa isotype control and mutants. Low or no specific binding to human samples tested. Humanized variable regions, and human IgG1 kappa constant regions. Chimeric versions and premium grade (endotoxin ≤0.05 EU/mg) are available.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card.

Description

PA007140: In Vivo Grade Recombinant Human IgG1-S267E L328F (SELF) Isotype Control Antibody

The in vivo grade recombinant human IgG1 kappa isotype control antibody (hIgG1 isotype control) was produced in CHO cells.

Background

Different from polyclonal “human IgG1 isotype control antibodies” purified from human myeloma serum, the recombinant human IgG1 isotype control (hIgG1 kappa isotype control antibody) and mutants are produced in mammalian cells (HEK293 and CHO cells), thus are monoclonal and there is no potential contamination of any other antibody isotype(s). The recombinant human IgG1 isotype control has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG1 kappa constant regions. The human IgG1 kappa isotype control and mutants such as Fc-silenced can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

A number of hIgG1 isotype control mutants are available. Most of the therapeutic antibody drugs carry the natural human IgG1 constant regions. There are some engineered mutants in the IgG1 Fc fragment. Changes to the IgG Fc sequence including glycosylation sites (such as N297A or N297G) have been used to modulate Fc functions. Even a single mutation may have both enhancement and abrogation effects on Fc binding though multiple mutation combinations are commonly used. We generated hIgG1 isotype control mutants, the same mutations as those in the therapeutic antibodies and candidates because such human IgG1 isotype control mutants are needed when clients test their engineered antibody candidates. For example, P329G substitution combined with L234A L235A (hIgG1 LALAPG, Fc silenced) was shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface. The silent Fc effctor functions human IgG1 isotype controls are useful for in vivo use.

The isotype controls are normally used to differentiate any non-specific background signal from the specific antibody signal, including the immunogenicity if used in animals. Most in vivo functional grade human IgG antibodies are used in mice for in vivo studies. Besides the antibody Complementarity-Determining Regions (CDRs), two human or humanized variable regions including the one in the human or humanized IgG isotype control do not necessarily induce the same immune response in mice because in some cases, even one amino acid difference can make difference. Mouse variable regions can be used to show the potential immunogenicity induced by the human variable regions in the human or humanized IgG antibodies to test. Thus, in some cases, it is better to use the mouse variable region in the human IgG isotype controls. We also produce and sell recombinant human IgG isotype controls from the popuplar mouse hybridoma clones, MOPC21 and C1.18.4, as well as recombinant human IgG1 kappa isotype controls against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, GFP, and unknown specificity.

Please contact us to ask for a quote for the Fc silenced human IgG1 isotype control mutants, hIgG1 isotype control and silent Fc mutants with Avi-, His-, and Flag-tags, and biotinylated hIgG1 isotype control and Fc silent mutants. A variety of conjugates (such as a fluorophore) with the human IgG1 isotype control and Fc effector-silent mutants are available.

Syd Labs provides the following in vivo grade recombinant IgG kappa isotype control antibody proteins:
In vivo grade recombinant human IgG2 isotype control antibody
In vivo grade recombinant human IgG4-S228P isotype control antibody
In vivo grade recombinant human IgG1 Fc protein
In vivo grade recombinant human IgG2 Fc protein
In vivo grade recombinant human IgG4-S228P Fc protein

Syd Labs provides the following in vivo grade recombinant human IgG1 kappa isotype control antibody mutants:
In vivo grade recombinant human IgG1-D265A isotype control antibody
In vivo grade recombinant human IgG1-N297A isotype control antibody
In vivo grade recombinant human IgG1-D265A N297A isotype control antibody
In vivo grade recombinant human IgG1-L234A L235A P329G (LALAPG) isotype control antibody

Syd Labs also provides a number of research grade antibody biosimilar proteins with competitive prices starting from $3/mg. Syd Labs introduces mutations to the antibody biosimilars including engineering or switching the isotypes or Fc engineering, such as Fc silent.

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Please remember our product information: In Vivo Grade Recombinant Human IgG1-S267E L328F (SELF) Isotype Control Antibody: PA007140 Syd Labs

Additional information

Ship from Country	USA
Size	1 mg, 25 mg, 5 mg

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