In vivo Grade Recombinant Anti-mouse CD25 Mouse Fab Fragment
$500.00
Recombinant anti-mouse CD25 mouse IgG2c monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion.
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Catalog No. | PA007156.r-1 |
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Product Name | In vivo Grade Recombinant Anti-mouse CD25 Mouse Fab Fragment |
Supplier Name | Syd Labs, Inc. |
Brand Name | Syd Labs |
Clone | PC61, PC61.5 or PC61.5.3. |
Applications | immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. |
Immunogen | IL-2-dependent cytolytic mouse T-cell clone B6.1. |
Form Of Antibody | 0.2 μM filtered solution of 1x PBS. |
Endotoxin | Less than 1 EU/mg of protein as determined by LAL method. |
Purity | >95% by SDS-PAGE under reducing conditions. |
Shipping | The in vivo grade recombinant anti-mouse CD25 mouse IgG2c kappa monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied. |
Note | Recombinant anti-mouse CD25 mouse IgG2c monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion. |
Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
Background
The in vivo grade recombinant anti-mouse CD25 monoclonal antibodies are based on the rat monoclonal antibody anti-CD25 (mouse) with the clone number of PC61, PC61.5 or PC61.5.3. The rat IgG1 lamda monoclonal antibody has been validated and reported for use in immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. In vivo depletion of mouse CD25(+)CD4(+)FoxP3(+) Treg cells using the rat PC61 monoclonal antibody is widely used to characterize the Treg function. The PC61 monoclonal antibody specifically binds to an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by the 3C7 and 7D4 monoclonal antibodies. By doing so, the antibody blocks binding of IL-2 to CD25.
PA007156.m2c: In Vivo Grade Recombinant Anti-mouse CD25 Mouse IgG2c Kappa Monoclonal Antibody
The in vivo grade recombinant anti-mouse CD25 mouse IgG2c kappa monoclonal antibody was produced in CHO cells.
Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
Syd Labs also provides the following research grade antibody biosimilar proteins:
Daclizumab Biosimilar, research grade, Anti-human CD25 Monoclonal Antibody
Additional information
Ship from Country | USA |
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Size | 1 mg |