Screening Monoclonal Hybridomas for Various Applications

Screening Monoclonal Hybridomas for Various Applications

De novo Antibody Sequencing Service

De novo Antibody Sequencing Service

Human and Monkey Monoclonal Antibody Production Services

Single B cell antibody cloning-based monoclonal antibody development services are also available on other species, especially rabbit, and mouse.

CatalogBS027
Grouped product items
Product Name Quantity
Custom Human Monoclonal Antibody Production (Catalog No.: BS027A)
Custom Monkey Monoclonal Antibody Production (Catalog No.: BS027B)

Syd Labs has successfully developed human and monkey monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the antibody variable regions (VH and VL) from single or clonal human and monkey B cells are cloned and sequenced. Recombinant rabbit monoclonal antibodies are produced, purified, and tested for various applications, such as drug development and diagnostics.

The procedure of custom human and monkey monoclonal antibody production services:

Phase I: Immunization and boosts of monkeys following the standard immunization protocol and ELISA screening of human or monkey polyclonal sera. The human or monkey positive respone to the antigen is critical to isolate high specificity and high affinity human and moneky monoclonal antibodies.

Phase II: PBMC isolation, detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, culturing single B cells in individual wells to secret antibodies, high throughput ELISA screening to isolate antigen-positive B cells. Supernatant (culture media) from ELISA-positive clones is sent to clients for evaluation of specific applications.

Phase III: Cloning and sequencing the antibody variable regions (VH and VL) from antigen specific B cells selected by clients, and transient production of recombinant human or monkey monoclonal antibodies in HEK 293 cells. Purified human or monkey monoclonal antibodies are sent to clients for confirmation and test of specific applications.

You provide: human or monkey polyclonal sera and PBMC, and the antigen.

Deliverable:
* About 80 ul supernatant from ELISA positive cells for evaluation;
* About 100 ug purified human or monkey monoclonal antibodies for evaluation;
* Amplified DNA fragments;
* Remaining antigens;
* Study report including ELISA results and antibody VH and VL sequences.

Clients have the choice for licensing antibodies to save cost and even make money.

Syd Labs also provides other monoclonal antibody development services:

Rabbit monoclonal antibody development services
Mouse monoclonal antibody development services
Rat monoclonal antibody development services
Camelid monoclonal antibody development services

References:

A Therapeutic Antibody for Cancer, Derived from Single Human B Cells. Bushey RT, Moody MA, Nicely NL, Haynes BF, Alam SM, Keir ST, Bentley RC, Roy Choudhury K, Gottlin EB, Campa MJ, Liao HX, Patz EF Jr. Cell Rep. 2016 May 17;15(7):1505-1513.

Exploring Human Antimicrobial Antibody Responses on a Single B Cell Level. Hofmann D, Lai JR. Clin Vaccine Immunol. 2017 May 5;24(5).

Generation of Human B-Cell Lines Dependent on CD40-Ligation and Interleukin-4. Banchereau J. Front Immunol. 2015 Feb 11;6:55.

Stable long-term cultures of self-renewing B cells and their applications. Kwakkenbos MJ, van Helden PM, Beaumont T, Spits H. Immunol Rev. 2016 Mar;270(1):65-77.

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