Heparinase I Enzyme
Conditions of optimal Heparinase I Enzyme performance should be determined experimentally by the investigator.
Custom service to make mixture of Heparinases (I, II, and/or III) is available.
Heparinase I enzyme (EC 220.127.116.11) cleaves heparin and to a lesser extent heparan sulfate (relative activity about 3:1) at the linkages between hexosamines and O-sulfated iduronic acids, yielding oligoaccharides (mainly disaccharides). In addition, Heparinase I cleaves heparin at the antithrombin III binding pentasaccharide domain.
Located in the extracellular matrix, the glycosaminoglycan of heparin plays important physiological roles in anticoagulation and angiogenesis. The acidic polysaccharide of Heparin consists of a heterogeneous disaccharide repeating unit of hexosamine and uronic acid (L-iduronic or D-glucuronic acid) connected through 1-4 linkages and modified with various functional groups.
The three heparinase enzymes (heparinase I, heparinase II, and heparinase III) produced by Flavobacterium heparinum specifically recognize and cleave at different sequences of heparin and heparan sulfate. Thus, the single and mixture of heparinases can be used for neutralization of heparin, detection and determination of plasma heparin, quality control in heparin manufacturing, and preparation of low molecular weight heparins, disaccharide or oligoaccharide from unfractionated heparin. If interested, please see more information about heparinases and their heparin-related applications.
BP000031-GD5: Recombinant Heparinase I from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >100 IU/mg. One international unit (IU) of recombinant Heparinase I is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0.
Purity >95% by SDS-PAGE.
Molecular weight: 42,508.
Form: In hepes buffer with 30% glycerol and 0.01M Ca2+.
Shipping: Recombinant heparinase I is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.