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Guaranteed Rat Monoclonal Antibody Development Services
The lowest prices are guaranteed. If you find any US company providing the same quality services with lower prices, we will return the difference to you.
The most comprehensive rat monoclonal antibody development services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.
BS009A: $4,600 for a standard 24-week hybridoma development program.
Phase I: Immunization and boosts of 5 rats following the standard immunization protocol.
Phase II: Fusion and ELISA screening. Out of 1,200 to 3,000 cells screened, about 300 microliter supernatants from 10 ELISA positive cells will be sent to customers for evaluation.
Phase III: Subcloning and expansion. All clones selected by the customer will be subcloned by limiting dilution, isotyped, and expanded.
You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.
* 50 ul of pre-immune serum;
* About 200 ul supernatant from 10 ELISA positive cells for evaluation;
* 1 positive hybridoma cell lines;
* Remaining antigens;
* ELISA results.
The highest quality standard in the field of mouse monoclonal antibody production services: guaranteed ELISA > 1:40,000.
Clients have the choice for licensing antibodies to save cost and even make money.
The prices will be adjusted for the following requirements:
a. Peptide (12-15 amino acids, >85%) design and synthesis +$250 for standard, +$450 for mono-phosphorylated, +$650 for di-phosphorylated, +$700 for mono-methylated (K or R site), +$650 for di-methylated (K or R site), +$700 for tri-methylated (K site), +$400 for acetylated;
b. Mouse monoclonal antibody production service for modified peptide: +$3,000 for phospho-specific, +$3,000 for methylation-specific, +$3,000 for acetylation-specific;
c. Synthesis of other antigens, +$variable;
d. Conjugation of antigens to protein carriers +$150;
e. Protein: DNA subcloning +$250, bacterial expression and purification +$1,800;
f. Additional boost immunization for higher ELISA titer of the antisera +$150/injection;
g. Additional positive hydridoma cell line +$400;
h. Antibody purification: antigen affinity purification +$350, protein A/G affinity purification +$300, ammonium sulfate precipitation +$250;
i. Guaranteed applications: Western blot +$3,500, IP +$3,500, IF +$4,500, ICC +$4,500, IHC +$5,000.