Conditions of optimal GM CSF Protein performance should be determined experimentally by the investigator.
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was first characterized as a growth factor that supports the in-vitro colony formation of granulocytes-macrophages progenitor cells. It is a pleiotropic cytokine and a member of a family of endogenous cytokines of the hematopoietic system. GM-CSF is produced as a response to immune or inflammatory stimuli by activated cells of the hematopoietic system such as T cells, B cells, macrophages, mast cells and also fibroblasts and alveolar epithelial cells. It plays an important role in regulating the proliferation, differentiation, survival and activation of hematopoietic cells such as granulocytes and monocytes, neutrophiles, basophiles and eosonophoiles, erythroid cells, megakaryocytes, and T cells. Bacterial endotoxins, TNF, Interleukin-1, and GM-CSF induce the synthesis of G-CSF.
BP000176-GD19: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein
Source: E. coli-derived.
Recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) protein is expressed with sequence (A18-E144) of human GM-CSF. It is a 14.6 kDa globular protein consisting of 128 amino acids (with the N-terminal methionine) containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.
N-terminal sequence analysis: (Met)-Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr-Gln-Pro-Trp-Glu-His.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10^7 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.01 ng/ug (0.1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 M NaCl, 5% Mannitol, pH 7.2.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other GM-CSF Proteins:
Recombinant human GM-CSF protein with His-tag
Recombinant human GM-CSF protein produced in HEK
Recombinant human GM-CSF protein produced in SF9
Recombinant human GM-CSF protein produced in yeast
Recombinant mouse GM-CSF protein
Recombinant rat GM-CSF protein
Recombinant porcine GM-CSF protein
Recombinant monkey GM-CSF protein
Recombinant canine GM-CSF protein
The recombinant human GM-CSF protein expressed in E. coli, such as molgramostin, is non-glycosylated, different from the natural glycoprotein and the recombinant human GM-CSF protein expressed in yeast cells, such as sargramostim.