FGF-2 / bFGF / FGF2 Protein
Conditions of optimal FGF-2 / bFGF / FGF2 Protein performance should be determined experimentally by the investigator.
FGF basic (bFGF/FGF-2/FGF2) is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic is produced by epithelial, tumor and other cell types. Involved in developmental processes and regulates differentiation, proliferation, and migration, FGF basic protein (bFGF/FGF-2/FGF2) is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic protein (bFGF/FGF-2/FGF2) has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic protein (bFGF/FGF-2/FGF2) binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways.
BP000030-GD4: Recombinant Human FGF Basic 147
Source: E. coli derived, Leu142-Ser288, with an N-terminal Gly, Accession# NP_001997
N-terminal sequence analysis: Gly-Leu142
Predicted molecular mass: 16.0 kDa
The FGF basic protein (bFGF/FGF-2/FGF2) activity is measured in a cell proliferation assay using NIH-3T3 mouse fibroblasts. The ED50 for this effect is less than 0.5 ng/ml.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
Formulation: 1.0 ug/ul from a 0.2 um filtered solution in PBS, pH 7.2.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt if stored at -20 to -70°C as supplied.
Other bFGF / FGF-2 / FGF2 Proteins:
Recombinant human FGF2 protein produced in HEK
Recombinant human FGF2 protein produced in SF9
Recombinant human FGF2 protein produced in plant
Recombinant mouse FGF2 protein
Recombinant rat FGF2 protein
Recombinant bovine FGF2 protein